Pig blastocyst-like structure models from embryonic stem cells.

Xiang, Jinzhu; Wang, Hanning; Shi, Bingbo; Li, Jiajun; Liu, Dong; Wang, Kaipeng; Wang, Zhuangfei; Min, Qiankun; Zhao, Chengchen; Pei, Duanqing

Xiang, Jinzhu; Wang, Hanning; Shi, Bingbo; Li, Jiajun; Liu, Dong; Wang, Kaipeng; Wang, Zhuangfei; Min, Qiankun; Zhao, Chengchen; Pei, Duanqing.

Afiliación

Xiang J; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Wang H; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Shi B; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Li J; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Liu D; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Wang K; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Wang Z; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Min Q; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
Zhao C; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China. zhaochengchen@westlake.edu.cn.
Pei D; Laboratory of Cell Fate Control, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China. peiduanqing@westlake.edu.cn.

Cell Discov ; 10(1): 72, 2024 Jul 02.

Article en En | MEDLINE | ID: mdl-38956027

ABSTRACT

ABSTRACT

Pluripotent stem cells have the potential to generate embryo models that can recapitulate developmental processes in vitro. Large animals such as pigs may also benefit from stem-cell-based embryo models for improving breeding. Here, we report the generation of blastoids from porcine embryonic stem cells (pESCs). We first develop a culture medium 4FIXY to derive pESCs. We develop a 3D two-step differentiation strategy to generate porcine blastoids from the pESCs. The resulting blastoids exhibit similar morphology, size, cell lineage composition, and single-cell transcriptome characteristics to blastocysts. These porcine blastoids survive and expand for more than two weeks in vitro under two different culture conditions. Large animal blastoids such as those derived from pESCs may enable in vitro modeling of early embryogenesis and improve livestock species' breeding practices.

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Cell Discov Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

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