Your browser doesn't support javascript.
loading
Mechanical factors influence ß-catenin localization and barrier properties.
Wu, Xi; Cesarovic, Nikola; Falk, Volkmar; Mazza, Edoardo; Giampietro, Costanza.
Afiliación
  • Wu X; ETH Zürich, DMAVT, Experimental Continuum Mechanics, Leonhardstrasse 21, Zurich 8092, Switzerland.
  • Cesarovic N; Department of Health Sciences and Technology, ETH Zürich, Leopold-Ruzicka-Weg 4, 8093 Zürich, Switzerland.
  • Falk V; Department of Cardiothoracic and Vascular Surgery, Deutsches Herzzentrum der Charité (DHZC), Augustenburger Platz 1, 13353 Berlin, Germany.
  • Mazza E; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany.
  • Giampietro C; Department of Health Sciences and Technology, ETH Zürich, Leopold-Ruzicka-Weg 4, 8093 Zürich, Switzerland.
Integr Biol (Camb) ; 162024 Jan 23.
Article en En | MEDLINE | ID: mdl-38952079
ABSTRACT
Mechanical forces are of major importance in regulating vascular homeostasis by influencing endothelial cell behavior and functions. Adherens junctions are critical sites for mechanotransduction in endothelial cells. ß-catenin, a component of adherens junctions and the canonical Wnt signaling pathway, plays a role in mechanoactivation. Evidence suggests that ß-catenin is involved in flow sensing and responds to tensional forces, impacting junction dynamics. The mechanoregulation of ß-catenin signaling is context-dependent, influenced by the type and duration of mechanical loads. In endothelial cells, ß-catenin's nuclear translocation and signaling are influenced by shear stress and strain, affecting endothelial permeability. The study investigates how shear stress, strain, and surface topography impact adherens junction dynamics, regulate ß-catenin localization, and influence endothelial barrier properties. Insight box Mechanical loads are potent regulators of endothelial functions through not completely elucidated mechanisms. Surface topography, wall shear stress and cyclic wall deformation contribute overlapping mechanical stimuli to which endothelial monolayer respond to adapt and maintain barrier functions. The use of custom developed flow chamber and bioreactor allows quantifying the response of mature human endothelial to well-defined wall shear stress and gradients of strain. Here, the mechanoregulation of ß-catenin by substrate topography, wall shear stress, and cyclic stretch is analyzed and linked to the monolayer control of endothelial permeability.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Mecánico / Uniones Adherentes / Mecanotransducción Celular / Células Endoteliales / Beta Catenina / Células Endoteliales de la Vena Umbilical Humana Límite: Humans Idioma: En Revista: Integr Biol (Camb) Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Mecánico / Uniones Adherentes / Mecanotransducción Celular / Células Endoteliales / Beta Catenina / Células Endoteliales de la Vena Umbilical Humana Límite: Humans Idioma: En Revista: Integr Biol (Camb) Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido