Protein Production at the 100L Scale.

Rangarajan, Sneha; von Berg, Grace; Allen, Heather; Mitchell, Carter

Rangarajan, Sneha; von Berg, Grace; Allen, Heather; Mitchell, Carter.

Afiliación

Rangarajan S; Kemp Proteins, Frederick, MD, USA. Sneha.rangarajan@kempproteins.com.
von Berg G; Kemp Proteins, Frederick, MD, USA.
Allen H; Kemp Proteins, Frederick, MD, USA.
Mitchell C; Kemp Proteins, Frederick, MD, USA.

Methods Mol Biol ; 2829: 195-202, 2024.

Article en En | MEDLINE | ID: mdl-38951335

ABSTRACT

ABSTRACT

The Baculovirus Expression Vector System (BEVS) has revolutionized the field of recombinant protein expression by enabling efficient and high yield production. The platform offers many advantages including manufacturing speed, flexible design, and scalability. In this chapter, we describe the methods including strategies and considerations to successfully optimize and scale-up using BEVS as a tool for production (Fig. 1). As an illustrative case study, we present an example focused on the production of a viral glycoprotein.

Asunto(s)

Baculoviridae; Vectores Genéticos; Proteínas Recombinantes; Baculoviridae/genética; Proteínas Recombinantes/genética; Proteínas Recombinantes/biosíntesis; Vectores Genéticos/genética; Animales; Humanos; Células Sf9

Palabras clave

Baculovirus expression vector system (BEVS); High titer stock (HTS); Hours post infection (hpi); Initial virus seed stock (P0); Insect cell culture; Optimization; Protein expression; Scale-up; Stirred tank reactor (STR)

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Baculoviridae / Vectores Genéticos Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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