Multi-Omics Analyses of Lettuce (<i>Lactuca sativa</i>) Reveals Primary Metabolism Reorganization Supporting Distinct Features of Secondary Metabolism Induced by Supplementing UV-A Radiation.

Zha, Lingyan; Wei, Shiwei; Huang, Danfeng; Zhang, Jingjin

Multi-Omics Analyses of Lettuce (Lactuca sativa) Reveals Primary Metabolism Reorganization Supporting Distinct Features of Secondary Metabolism Induced by Supplementing UV-A Radiation.

Zha, Lingyan; Wei, Shiwei; Huang, Danfeng; Zhang, Jingjin.

Afiliación

Zha L; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Wei S; Shanghai Agrobiological Gene Center, Shanghai 201106, China.
Huang D; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Zhang J; School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

J Agric Food Chem ; 72(28): 15498-15511, 2024 Jul 17.

Article en En | MEDLINE | ID: mdl-38950542

ABSTRACT

ABSTRACT

UV can serve as an effective light spectrum for regulating plant secondary metabolites, while relevant studies on UV-A are much less extensive than those on UV-B. A comprehensive understanding of the selective effects of UV-A on different secondary metabolites and the specific features of primary metabolism that drive these effects is still lacking. To address this knowledge gap, we conducted a study to analyze the dynamic changes in the metabolome and transcriptome of lettuce leaves irradiated with red plus UV-A light (monochromatic red light as control). Generally, UV-A promoted the synthesis of most phenylpropanoids and terpenoids originating from the shikimate and methylerythritol phosphate (MEP) pathway in plastids but sacrificed the synthesis of terpenoids derived from the mevalonate (MVA) pathway, particularly sesquiterpenes. Increased precursors supply for the shikimate and MEP pathway under UV-A was directly supported by the activation of the Calvin-Benson cycle and phosphoenolpyruvate transport. Whereas, along with phosphoenolpyruvate transport, the TCA cycle was restrained, causing deprivation of the MVA pathway precursor. In addition, UV-A also activated the plastidic oxidative branch of the pentose phosphate pathway, photorespiration, and malate shuttle, to ensure a sufficient supply of nitrogen, circulation homeostasis of the Calvin-Benson cycle, and energy balance, thus indirectly supporting UV-A-induced specific secondary metabolic output. This study provides a comprehensive framework for understanding the flexible primary-secondary metabolism interactions that are able to produce specific metabolites favorable for adaptation to environmental stimuli.

Asunto(s)

Lactuca; Hojas de la Planta; Metabolismo Secundario; Rayos Ultravioleta; Lactuca/metabolismo; Lactuca/efectos de la radiación; Lactuca/química; Lactuca/genética; Lactuca/crecimiento & desarrollo; Metabolismo Secundario/efectos de la radiación; Hojas de la Planta/metabolismo; Hojas de la Planta/efectos de la radiación; Hojas de la Planta/química; Proteínas de Plantas/metabolismo; Proteínas de Plantas/genética; Metaboloma/efectos de la radiación; Regulación de la Expresión Génica de las Plantas/efectos de la radiación; Multiómica

Palabras clave

malate shuttle; metabolic network; photorespiration; primary metabolism; specialized metabolism; ultraviolet

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rayos Ultravioleta / Hojas de la Planta / Lactuca / Metabolismo Secundario Idioma: En Revista: J Agric Food Chem Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

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