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Quantifying Viable M. tuberculosis Safely Obviating the Need for High Containment Facilities.
Mtafya, Bariki; Musisi, Emmanuel; Qwaray, Paschal; Sichone, Emanuel; Walbaum, Natasha; Ntinginya, Nyanda Elias; Gillespie, Stephen H; Sabiiti, Wilber.
Afiliación
  • Mtafya B; National Institute for Medical Research - Mbeya Medical Research Centre, Mbeya, Tanzania.
  • Musisi E; Infectious Diseases Research Collaboration, Kampala, Uganda.
  • Qwaray P; Adroit Biomedical & Bioentreprenuership Research Services, Kampala, Uganda.
  • Sichone E; National Institute for Medical Research - Mbeya Medical Research Centre, Mbeya, Tanzania.
  • Walbaum N; National Institute for Medical Research - Mbeya Medical Research Centre, Mbeya, Tanzania.
  • Ntinginya NE; Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, UK.
  • Gillespie SH; National Institute for Medical Research - Mbeya Medical Research Centre, Mbeya, Tanzania.
  • Sabiiti W; Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, UK.
Methods Mol Biol ; 2833: 145-152, 2024.
Article en En | MEDLINE | ID: mdl-38949708
ABSTRACT
Mycobacterium tuberculosis is an infectious pathogen that requires biosafety level-3 laboratory for handling. The risk of transmission is high to laboratory staff, and to manage the organism safely, it is necessary to construct high containment laboratory facilities at great expense. This limits the application of tuberculosis diagnostics to areas where there is insufficient capital to invest in laboratory infrastructure. In this method, we describe a process of inactivating sputum samples by either heat or guanidine thiocyanate (GTC) that renders them safe without affecting the quantification of viable bacteria. This method eliminates the need for level 3 containment laboratory for the tuberculosis molecular bacterial load assay (TB-MBLA) and is applicable in low- and middle-income countries.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esputo / Tiocianatos / Contención de Riesgos Biológicos / Mycobacterium tuberculosis Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Tanzania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esputo / Tiocianatos / Contención de Riesgos Biológicos / Mycobacterium tuberculosis Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Tanzania Pais de publicación: Estados Unidos