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Liver bioprinting within a novel support medium with functionalized spheroids, hepatic vein structures, and enhanced post-transplantation vascularization.
Jiang, Zhuoran; Jin, Bao; Liang, Zhu; Wang, Yinhan; Ren, Shuai; Huang, Yongfa; Li, Changcan; Sun, Hang; Li, Yunzhu; Liu, Li; Li, Nianlin; Wang, Jinzhuo; Cui, Zhanfeng; Huang, Pengyu; Yang, Huayu; Mao, Yilei; Ye, Hua.
Afiliación
  • Jiang Z; Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, UK.
  • Jin B; Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China.
  • Liang Z; Target Discovery Institute, Centre for Medicines Discovery, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7FZ, UK; Chinese Academy of Medical Sciences (CAMS), CAMS Oxford Institute (COI), Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7FZ, UK.
  • Wang Y; Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China.
  • Ren S; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, UK.
  • Huang Y; Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China.
  • Li C; Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China.
  • Sun H; Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China.
  • Li Y; Department of Plastic and Reconstructive Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China.
  • Liu L; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, UK.
  • Li N; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, UK.
  • Wang J; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, UK.
  • Cui Z; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, UK; The Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Suzhou, 215123, China.
  • Huang P; Engineering Research Center of Pulmonary and Critical Care Technology and Device (MOE of China), Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, China. Electronic address: huangpengyu@yeah.net.
  • Yang H; Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China. Electronic address: dolphinyahy@hotmail.com.
  • Mao Y; Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital, PUMC & Chinese Academy of Medical Sciences (CAMS), Beijing, China. Electronic address: pumch-liver@hotmail.com.
  • Ye H; Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, UK; The Oxford Suzhou Centre for Advanced Research (OSCAR), University of Oxford, Suzhou, 215123, China. Electronic address: hua.ye@eng.ox.ac.uk.
Biomaterials ; 311: 122681, 2024 Dec.
Article en En | MEDLINE | ID: mdl-38944968
ABSTRACT
Cell-laden bioprinting is a promising biofabrication strategy for regenerating bioactive transplants to address organ donor shortages. However, there has been little success in reproducing transplantable artificial organs with multiple distinctive cell types and physiologically relevant architecture. In this study, an omnidirectional printing embedded network (OPEN) is presented as a support medium for embedded 3D printing. The medium is state-of-the-art due to its one-step preparation, fast removal, and versatile ink compatibility. To test the feasibility of OPEN, exceptional primary mouse hepatocytes (PMHs) and endothelial cell line-C166, were used to print hepatospheroid-encapsulated-artificial livers (HEALs) with vein structures following predesigned anatomy-based printing paths in OPEN. PMHs self-organized into hepatocyte spheroids within the ink matrix, whereas the entire cross-linked structure remained intact for a minimum of ten days of cultivation. Cultivated HEALs maintained mature hepatic functions and marker gene expression at a higher level than conventional 2D and 3D conditions in vitro. HEALs with C166-laden vein structures promoted endogenous neovascularization in vivo compared with hepatospheroid-only liver prints within two weeks of transplantation. Collectively, the proposed platform enables the manufacture of bioactive tissues or organs resembling anatomical architecture, and has broad implications for liver function replacement in clinical applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esferoides Celulares / Neovascularización Fisiológica / Hepatocitos / Bioimpresión / Impresión Tridimensional / Venas Hepáticas / Hígado Límite: Animals Idioma: En Revista: Biomaterials Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esferoides Celulares / Neovascularización Fisiológica / Hepatocitos / Bioimpresión / Impresión Tridimensional / Venas Hepáticas / Hígado Límite: Animals Idioma: En Revista: Biomaterials Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos