Your browser doesn't support javascript.
loading
Genetic mapping of regions associated with root system architecture in rice using MutMap QTL-seq.
Magar, Nakul D; Barbadikar, Kalyani M; Reddy, Vishal; Revadi, Padmashree; Guha, Pritam; Gangatire, Dhiraj; Balakrishnan, Divya; Sharma, Shailendra; Madhav, M Sheshu; Sundaram, Raman M.
Afiliación
  • Magar ND; ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India; Chaudhary Charan Singh University, Meerut, 250005, India.
  • Barbadikar KM; ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India. Electronic address: Kalyaniaau@gmail.com.
  • Reddy V; ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India.
  • Revadi P; ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India.
  • Guha P; ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India.
  • Gangatire D; ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India.
  • Balakrishnan D; ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India.
  • Sharma S; Chaudhary Charan Singh University, Meerut, 250005, India.
  • Madhav MS; ICAR-Central Tobacco Research Institute, Rajahmundry, 533106, India.
  • Sundaram RM; ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India.
Plant Physiol Biochem ; 213: 108836, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38941724
ABSTRACT
The root system architecture is an important complex trait in rice. With changing climatic conditions and soil nutrient deficiencies, there is an immediate need to breed nutrient-use-efficient rice varieties with robust root system architectural (RSA) traits. To map the genomic regions associated with crucial component traits of RSA viz. root length and root volume, a biparental F2 mapping population was developed using TI-128, an Ethyl Methane Sulphonate (EMS) mutant of a mega variety BPT-5204 having high root length (RL) and root volume (RV) with wild type BPT-5204. Extreme bulks having high RL and RV and low RL and RV were the whole genome re-sequenced along with parents. Genetic mapping using the MutMap QTL-Seq approach elucidated two genomic intervals on Chr.12 (3.14-3.74 Mb, 18.11-20.85 Mb), and on Chr.2 (23.18-23.68 Mb) as potential regions associated with both RL and RV. The Kompetitive Allele Specific PCR (KASP) assays for SNPs with delta SNP index near 1 were associated with higher RL and RV in the panel of sixty-two genotypes varying in root length and volume. The KASP_SNPs viz. Chr12_S4 (C→T; Chr123243938), located in the 3' UTR region of LOC_Os12g06670 encoding a protein kinase domain-containing protein and Chr2_S6 (C→T; Chr223181622) present upstream in the regulator of chromosomal condensation protein LOC_Os2g38350. Validation of these genes using qRT-PCR and in-silico studies using various online tools and databases revealed higher expression in TI-128 as compared to BPT- 5204 at the seedling and panicle initiation stages implying the functional role in enhancing RL and RV.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Mapeo Cromosómico / Raíces de Plantas / Sitios de Carácter Cuantitativo Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Francia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oryza / Mapeo Cromosómico / Raíces de Plantas / Sitios de Carácter Cuantitativo Idioma: En Revista: Plant Physiol Biochem Asunto de la revista: BIOQUIMICA / BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Francia