Your browser doesn't support javascript.
loading
Functional validation of AaCaM3 response to high temperature stress in Amorphophallus albus.
Niu, Yi; Zhou, Zixuan; Yue, Zhenyu; Zhang, Xiaofei; Jiang, Xuekuan; Hu, Lingyu; Liu, Quanshuo; Zhang, Xu; Dong, Kun.
Afiliación
  • Niu Y; Yibin Academy of Southwest University, Yibin, China. niuy2001134@163.com.
  • Zhou Z; College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China. niuy2001134@163.com.
  • Yue Z; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Southwest University, Chongqing, China. niuy2001134@163.com.
  • Zhang X; College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
  • Jiang X; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Southwest University, Chongqing, China.
  • Hu L; College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
  • Liu Q; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Southwest University, Chongqing, China.
  • Zhang X; College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
  • Dong K; Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Southwest University, Chongqing, China.
BMC Plant Biol ; 24(1): 615, 2024 Jun 28.
Article en En | MEDLINE | ID: mdl-38937722
ABSTRACT
Amorphophallus is a perennial monocotyledonous herbaceous plant native to the southwestern region of China, widely used in various fields such as food processing, biomedicine and chemical agriculture. However, Amorphophallus is a typical thermolabile plant, and the continuous high temperature in summer have seriously affected the growth, development and economic yield of Amorphophallus in recent years. Calmodulin (CaM), a Ca2+ sensor ubiquitous in eukaryotes, is the most important multifunctional receptor protein in plant cells, which affects plant stress resistance by participating in the activities of a variety of signaling molecules. In this study, the key gene AaCaM3 for the Ca2+-CaM regulatory pathway was obtained from A. albus, the sequence analysis confirmed that it is a typical calmodulin. The qRT-PCR results demonstrated that with the passage of heat treatment time, the expression of AaCaM3 was significantly upregulated in A. albus leaves. Subcellular localization analysis revealed that AaCaM3 localized on the cytoplasm and nucleus. Meanwhile, heterologous transformation experiments have shown that AaCaM3 can significantly improve the heat tolerance of Arabidopsis under heat stress. The promoter region of AaCaM3 was sequenced 1,338 bp by FPNI-PCR and GUS staining assay showed that the promoter of AaCaM3 was a high-temperature inducible promoter. Yeast one-hybrid analysis and Luciferase activity reporting system analysis showed that the AaCaM3 promoter may interact with AaHSFA1, AaHSFA2c, AaHSP70, AaDREB2a and AaDREB2b. In conclusion, this study provides new ideas for further improving the signal transduction network of high-temperature stress in Amorphophallus.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Calmodulina / Arabidopsis Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Calmodulina / Arabidopsis Idioma: En Revista: BMC Plant Biol Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido