Compound-Specific Carbon, Nitrogen, and Hydrogen Isotope Analysis to Characterize Aerobic Biodegradation of 2,3-Dichloroaniline by a Mixed Enrichment Culture.

Suchana, Shamsunnahar; Araujo, Sofia Pimentel; Lomheim, Line; Mack, E Erin; Spain, Jim C; Edwards, Elizabeth; Passeport, Elodie

Suchana, Shamsunnahar; Araujo, Sofia Pimentel; Lomheim, Line; Mack, E Erin; Spain, Jim C; Edwards, Elizabeth; Passeport, Elodie.

Afiliación

Suchana S; Department of Civil & Mineral Engineering, University of Toronto, 35 Saint George Street, Toronto, Ontario M5S 1A4, Canada.
Araujo SP; Department of Chemical Engineering & Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario M5S 3E5, Canada.
Lomheim L; Department of Chemical Engineering & Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario M5S 3E5, Canada.
Mack EE; Corteva Remediation Group, Wilmington, Delaware 19805, United States.
Spain JC; Center for Environmental Diagnostics and Bioremediation, University of West Florida, Pensacola, Florida 32514-5751, United States.
Edwards E; Department of Chemical Engineering & Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario M5S 3E5, Canada.
Passeport E; Department of Civil & Mineral Engineering, University of Toronto, 35 Saint George Street, Toronto, Ontario M5S 1A4, Canada.

Environ Sci Technol ; 58(27): 12042-12050, 2024 Jul 09.

Article en En | MEDLINE | ID: mdl-38934904

ABSTRACT

ABSTRACT

Compound-specific isotope analysis (CSIA) is an established tool to track the in situ transformation of organic chemicals at contaminated sites. In this work, we evaluated the potential of multi-element CSIA to assess biodegradation of 2,3-dichloroaniline (2,3-DCA), which is a major industrial feedstock. Using controlled laboratory experiments, we determined, for the first time, negligible carbon (<0.5) and hydrogen (<10) isotope fractionation and a significant inverse nitrogen isotope fractionation (>10) during aerobic 2,3-DCA biodegradation by a mixed enrichment culture. The tentative identification of a glutamate conjugate of 2,3-DCA as a reaction intermediate indicates that the initial multistep enzymatic reaction may be rate-limiting. The formation of the glutamate adduct would increase the bond energy at the N atom, thus likely explaining the observed inverse N isotope fractionation. The corresponding nitrogen enrichment factor was +6.8 ± 0.6. This value was applied to investigate the in situ 2,3-DCA biodegradation at a contaminated site where the carbon and nitrogen isotope signatures from field samples suggested similar aerobic processes by native microorganisms. Under the assumption of the applicability of the Rayleigh model in a pilot wetland treating contaminated groundwater, the extent of biodegradation was estimated to be up to 80-90%. This study proposes multi-element CSIA as a novel application to study 2,3-DCA fate in groundwater and surface water and provides insights into biodegradation pathways.

Asunto(s)

Biodegradación Ambiental; Compuestos de Anilina/metabolismo; Isótopos de Carbono; Isótopos de Nitrógeno; Aerobiosis; Nitrógeno/metabolismo

Palabras clave

CSIA; biodegradation; bulk enrichment factor; dichloroaniline; stable isotopes; transformation product

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biodegradación Ambiental Idioma: En Revista: Environ Sci Technol Año: 2024 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos

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