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Assessing Predictive Value of SARS-CoV-2 Epitope-Specific CD8+ T-Cell Response in Patients with Severe Symptoms.
Martín-Martín, Cristina; Del Riego, Estefanía Salgado; Castiñeira, Jose R Vidal; Zapico-Gonzalez, Maria Soledad; Rodríguez-Pérez, Mercedes; Corte-Iglesias, Viviana; Saiz, Maria Laura; Diaz-Bulnes, Paula; Escudero, Dolores; Suárez-Alvarez, Beatriz; López-Larrea, Carlos.
Afiliación
  • Martín-Martín C; Translational Immunology, Health Research Institute of the Principality of Asturias (ISPA), Avenida de Roma S/N, 33011 Oviedo, Spain.
  • Del Riego ES; Service of Intensive Medicine, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain.
  • Castiñeira JRV; Health Research Institute of the Principality of Asturias (ISPA), Avenida de Roma S/N, 33011 Oviedo, Spain.
  • Zapico-Gonzalez MS; Translational Immunology, Health Research Institute of the Principality of Asturias (ISPA), Avenida de Roma S/N, 33011 Oviedo, Spain.
  • Rodríguez-Pérez M; Immunology Department, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain.
  • Corte-Iglesias V; Microbiology Department, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain.
  • Saiz ML; Microbiology Department, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain.
  • Diaz-Bulnes P; Translational Microbiology, Health Research Institute of Principado de Asturias (ISPA), 33011 Oviedo, Spain.
  • Escudero D; Translational Immunology, Health Research Institute of the Principality of Asturias (ISPA), Avenida de Roma S/N, 33011 Oviedo, Spain.
  • Suárez-Alvarez B; Immunology Department, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain.
  • López-Larrea C; Translational Immunology, Health Research Institute of the Principality of Asturias (ISPA), Avenida de Roma S/N, 33011 Oviedo, Spain.
Vaccines (Basel) ; 12(6)2024 Jun 18.
Article en En | MEDLINE | ID: mdl-38932408
ABSTRACT
Specific T cell responses against SARS-CoV-2 provided an overview of acquired immunity during the pandemic. Anti-SARS-CoV-2 immunity determines the severity of acute illness, but also might be related to the possible persistence of symptoms (long COVID). We retrospectively analyzed ex vivo longitudinal CD8+ T cell responses in 26 COVID-19 patients diagnosed with severe disease, initially (1 month) and long-term (10 months), and in a cohort of 32 vaccinated healthcare workers without previous SARS-CoV-2 infection. We used peptide-human leukocyte antigen (pHLA) dextramers recognizing 26 SARS-CoV-2-derived epitopes of viral and other non-structural proteins. Most patients responded to at least one of the peptides studied, mainly derived from non-structural ORF1ab proteins. After 10 months follow-up, CD8+ T cell responses were maintained at long term and reaction against certain epitopes (A*0101-ORF1ab1637) was still detected and functional, showing a memory-like phenotype (CD127+ PD-1+). The total number of SARS-CoV-2-specific CD8+ T cells was significantly associated with protection against long COVID in these patients. Compared with vaccination, infected patients showed a less effective immune response to spike protein-derived peptides restricted by HLA. So, the A*0101-S865 and A*2402-S1208 dextramers were only recognized in vaccinated individuals. We conclude that initial SARS-CoV-2-specific CD8+ T cell response could be used as a marker to understand the evolution of severe disease and post-acute sequelae after SARS-CoV-2 infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vaccines (Basel) Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vaccines (Basel) Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza