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Enhancing Dengue Virus Production and Immunogenicity with Celcradle™ Bioreactor: A Comparative Study with Traditional Cell Culture Methods.
Guo, Hongxia; Ding, Xiaoyan; Hua, Dong; Liu, Minchi; Yang, Maocheng; Gong, Yuanxin; Ye, Nan; Chen, Xiaozhong; He, Jiuxiang; Zhang, Yu; Xu, Xiaofeng; Li, Jintao.
Afiliación
  • Guo H; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • Ding X; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • Hua D; Department of Pediatrics, Ludwig-Maximilians University of Munich, 80337 Munich, Germany.
  • Liu M; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • Yang M; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • Gong Y; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • Ye N; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • Chen X; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • He J; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • Zhang Y; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • Xu X; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
  • Li J; Department of Biosafety, School of Basic Medicine, Army Medical University, Chongqing 400038, China.
Vaccines (Basel) ; 12(6)2024 May 22.
Article en En | MEDLINE | ID: mdl-38932292
ABSTRACT
The dengue virus, the primary cause of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome, is the most widespread mosquito-borne virus worldwide. In recent decades, the prevalence of dengue fever has increased markedly, presenting substantial public health challenges. Consequently, the development of an efficacious vaccine against dengue remains a critical goal for mitigating its spread. Our research utilized Celcradle™, an innovative tidal bioreactor optimized for high-density cell cultures, to grow Vero cells for dengue virus production. By maintaining optimal pH levels (7.0 to 7.4) and glucose concentrations (1.5 g/L to 3.5 g/L) during the proliferation of cells and viruses, we achieved a peak Vero cell count of approximately 2.46 × 109, nearly ten times the initial count. The use of Celcradle™ substantially decreased the time required for cell yield and virus production compared to conventional Petri dish methods. Moreover, our evaluation of the immunogenicity of the Celcradle™-produced inactivated DENV4 through immunization of mice revealed that sera from these mice demonstrated cross-reactivity with DENV4 cultured in Petri dishes and showed elevated antibody titers compared to those from mice immunized with virus from Petri dishes. These results indicate that the dengue virus cultivated using the Celcradle™ system exhibited enhanced immunogenicity relative to that produced in traditional methods. In conclusion, our study highlights the potential of the Celcradle™ bioreactor for large-scale production of inactivated dengue virus vaccines, offering significant promise for reducing the global impact of dengue virus infections and accelerating the development of effective vaccination strategies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vaccines (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vaccines (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza