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LINC00662 Promotes Aggressive Traits by Modulating OCT4 Expression through miR-335-5p in Gallbladder Cancer Cells.
Pérez-Moreno, Pablo; Riquelme, Ismael; Bizama, Carolina; Vergara-Gómez, Luis; Tapia, Julio C; Brebi, Priscilla; García, Patricia; Roa, Juan Carlos.
Afiliación
  • Pérez-Moreno P; Programa de Comunicación Celular en Cáncer, Facultad de Medicina Clínica Alemana, Universidad del Desarrollo, Santiago 7780272, Chile.
  • Riquelme I; Institute of Biomedical Sciences, Faculty of Health Sciences, Universidad Autónoma de Chile, Temuco 4810101, Chile.
  • Bizama C; Millenium Institute on Immunology and Immunotherapy (IMII), Centro de Prevención y Control de Cancer (CECAN), Department of Pathology, School of Medicine, Pontificia Universidad Católica de Chile, Santiago 8380000, Chile.
  • Vergara-Gómez L; Centre of Excellence in Translational Medicine (CEMT), Scientific and Technological Bioresource Nucleus (BIOREN), Biomedicine and Translational Research Lab, Universidad de la Frontera, Temuco 4810296, Chile.
  • Tapia JC; Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile.
  • Brebi P; Laboratory of Integrative Biology (LIBi), Millennium Institute on Immunology and Immunotherapy (MIII), Center for Excellence in Translational Medicine-Scientific and Technological Bioresource Nucleus (CEMT-BIOREN), Universidad de La Frontera, Temuco 4810296, Chile.
  • García P; Millenium Institute on Immunology and Immunotherapy (IMII), Centro de Prevención y Control de Cancer (CECAN), Department of Pathology, School of Medicine, Pontificia Universidad Católica de Chile, Santiago 8380000, Chile.
  • Roa JC; Millenium Institute on Immunology and Immunotherapy (IMII), Centro de Prevención y Control de Cancer (CECAN), Department of Pathology, School of Medicine, Pontificia Universidad Católica de Chile, Santiago 8380000, Chile.
Int J Mol Sci ; 25(12)2024 Jun 19.
Article en En | MEDLINE | ID: mdl-38928444
ABSTRACT
Long non-coding RNAs (lncRNAs) are nucleotide sequences that participate in different biological processes and are associated with different pathologies, including cancer. Long intergenic non-protein-coding RNA 662 (LINC00662) has been reported to be involved in different cancers, including colorectal, prostate, and breast cancer. However, its role in gallbladder cancer has not yet been described. In this article, we hypothesize that LINC00662 has an important role in the acquisition of aggressiveness traits such as a stem-like phenotype, invasion, and chemoresistance in gallbladder cancer. Here, we show that LINC00662 is associated with larger tumor size and lymph node metastasis in patients with gallbladder cancer. Furthermore, we show that the overexpression of LINC00662 promotes an increase in CD133+/CD44+ cell populations and the expression of stemness-associated genes. LINC00662 promotes greater invasive capacity and the expression of genes associated with epithelial-mesenchymal transition. In addition, the expression of LINC00662 promotes resistance to cisplatin and 5-fluorouracil, associated with increased expression of chemoresistance-related ATP-binding cassette (ABC) transporters in gallbladder cancer (GBC) cell lines. Finally, we show that the mechanism by which LINC00662 exerts its function is through a decrease in microRNA 335-5p (miR-335-5p) and an increase in octamer-binding transcription factor 4 (OCT4) in GBC cells. Thus, our data allow us to propose LINC00662 as a biomarker of poor prognosis and a potential therapeutic target for patients with GBC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Neoplásica de la Expresión Génica / MicroARNs / Factor 3 de Transcripción de Unión a Octámeros / ARN Largo no Codificante / Neoplasias de la Vesícula Biliar Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación Neoplásica de la Expresión Génica / MicroARNs / Factor 3 de Transcripción de Unión a Octámeros / ARN Largo no Codificante / Neoplasias de la Vesícula Biliar Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza