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Rapid Movement of Palmitoleic Acid from Phosphatidylcholine to Phosphatidylinositol in Activated Human Monocytes.
Bermúdez, Miguel A; Garrido, Alvaro; Pereira, Laura; Garrido, Teresa; Balboa, María A; Balsinde, Jesús.
Afiliación
  • Bermúdez MA; Bioactive Lipids and Lipidomics Core, IBGM, CSIC-UVA, 47003 Valladolid, Spain.
  • Garrido A; Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, Spain.
  • Pereira L; Bioactive Lipids and Lipidomics Core, IBGM, CSIC-UVA, 47003 Valladolid, Spain.
  • Garrido T; Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, Spain.
  • Balboa MA; Bioactive Lipids and Lipidomics Core, IBGM, CSIC-UVA, 47003 Valladolid, Spain.
  • Balsinde J; Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, Spain.
Biomolecules ; 14(6)2024 Jun 15.
Article en En | MEDLINE | ID: mdl-38927110
ABSTRACT
This work describes a novel route for phospholipid fatty acid remodeling involving the monounsaturated fatty acid palmitoleic acid. When administered to human monocytes, palmitoleic acid rapidly incorporates into membrane phospholipids, notably into phosphatidylcholine (PC). In resting cells, palmitoleic acid remains within the phospholipid pools where it was initially incorporated, showing no further movement. However, stimulation of the human monocytes with either receptor-directed (opsonized zymosan) or soluble (calcium ionophore A23187) agonists results in the rapid transfer of palmitoleic acid moieties from PC to phosphatidylinositol (PI). This is due to the activation of a coenzyme A-dependent remodeling route involving two different phospholipase A2 enzymes that act on different substrates to generate free palmitoleic acid and lysoPI acceptors. The stimulated enrichment of specific PI molecular species with palmitoleic acid unveils a hitherto-unrecognized pathway for lipid turnover in human monocytes which may play a role in regulating lipid signaling during innate immune activation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilcolinas / Fosfatidilinositoles / Ácidos Grasos Monoinsaturados / Monocitos Límite: Humans Idioma: En Revista: Biomolecules Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidilcolinas / Fosfatidilinositoles / Ácidos Grasos Monoinsaturados / Monocitos Límite: Humans Idioma: En Revista: Biomolecules Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Suiza