Induction of acute silkworm hemolymph melanization by Staphylococcus aureus treated with peptidoglycan-degrading enzymes.
Drug Discov Ther
; 18(3): 194-198, 2024 Jul 09.
Article
en En
| MEDLINE
| ID: mdl-38925960
ABSTRACT
Staphylococcus aureus, a Gram-positive bacterium, causes inflammatory skin diseases, such as atopic dermatitis, and serious systemic diseases, such as sepsis. In the skin and nasal environment, peptidoglycan (PGN)-degrading enzymes, including lysozyme and lysostaphin, affects S. aureus PGN. However, the effects of PGN-degrading enzymes on the acute innate immune-inducing activity of S. aureus have not yet been investigated. In this study, we demonstrated that PGN-degrading enzymes induce acute silkworm hemolymph melanization by S. aureus. Insoluble fractions of S. aureus treated with lysozyme, lysostaphin, or both enzymes, were prepared. Melanization of the silkworm hemolymph caused by the injection of these insoluble fractions was higher than that of S. aureus without enzyme treatment. These results suggest that structural changes in S. aureus PGN caused by PGN-degrading enzymes affect the acute innate immune response in silkworms.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bombyx
/
Staphylococcus aureus
/
Peptidoglicano
/
Hemolinfa
/
Muramidasa
/
Inmunidad Innata
Límite:
Animals
Idioma:
En
Revista:
Drug Discov Ther
Año:
2024
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Japón