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Rapid evolutionary tuning of endospore quantity versus quality trade-off via a phase-variable contingency locus.
Kim, Tom Dongmin; Khanal, Sadhana; Bäcker, Leonard E; Lood, Cédric; Kerremans, Alison; Gorivale, Sayali; Begyn, Katrien; Cambré, Alexander; Rajkovic, Andreja; Devlieghere, Frank; Heyndrickx, Marc; Michiels, Chris; Duitama, Jorge; Aertsen, Abram.
Afiliación
  • Kim TD; Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven, 3001 Leuven, Belgium.
  • Khanal S; Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven, 3001 Leuven, Belgium.
  • Bäcker LE; Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven, 3001 Leuven, Belgium.
  • Lood C; Department of Biology, University of Oxford, Oxford OX1 3SZ, UK.
  • Kerremans A; Department of Biosystems, Faculty of Bioscience Engineering, KU Leuven, 3001 Leuven, Belgium.
  • Gorivale S; Department of Biosystems, Faculty of Bioscience Engineering, KU Leuven, 3001 Leuven, Belgium.
  • Begyn K; Department of Food Technology, Safety and Health, Part of Food2Know, Faculty Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
  • Cambré A; Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven, 3001 Leuven, Belgium.
  • Rajkovic A; Department of Food Technology, Safety and Health, Part of Food2Know, Faculty Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
  • Devlieghere F; Department of Food Technology, Safety and Health, Part of Food2Know, Faculty Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.
  • Heyndrickx M; ILVO-Flanders Research Institute for Agriculture, Fishery and Food, Technology and Food Science, Unit-Food Safety, 9090 Melle, Belgium; Department of Pathobiology, Pharmacology and Zoological Medicine, Faculty of Veterinary Medicine, Ghent University, 9820 Merelbeke, Belgium.
  • Michiels C; Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven, 3001 Leuven, Belgium; Leuven Food Science and Nutritional Research Centre (LeFoRCe), Department of Microbial and Molecular Systems (M2S), Faculty of Bioscience Engineering, KU Leuven, 3001 Leuven, Belgium.
  • Duitama J; Systems and Computing Engineering Department, Universidad de los Andes, 111711 Bogotá, Colombia.
  • Aertsen A; Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven, 3001 Leuven, Belgium. Electronic address: abram.aertsen@kuleuven.be.
Curr Biol ; 34(14): 3077-3085.e5, 2024 Jul 22.
Article en En | MEDLINE | ID: mdl-38925118
ABSTRACT
The UV resistance of bacterial endospores is an important quality supporting their survival in inhospitable environments and therefore constitutes an essential driver of the ecological success of spore-forming bacteria. Nevertheless, the variability and evolvability of this trait are poorly understood. In this study, directed evolution and genetics approaches revealed that the Bacillus cereus pdaA gene (encoding the endospore-specific peptidoglycan-N-acetylmuramic acid deacetylase) serves as a contingency locus in which the expansion and contraction of short tandem repeats can readily compromise (PdaAOFF) or restore (PdaAON) the pdaA open reading frame. Compared with B. cereus populations in the PdaAON state, populations in the PdaAOFF state produced a lower yield of viable endospores but endowed them with vastly increased UV resistance. Moreover, selection pressures based on either quantity (i.e., yield of viable endospores) or quality (i.e., UV resistance of viable endospores) aspects could readily shift populations between PdaAON and PdaAOFF states, respectively. Bioinformatic analysis also revealed that pdaA homologs within the Bacillus and Clostridium genera are often equipped with several short tandem repeat regions, suggesting a wider implementation of the pdaA-mediated phase variability in other sporeformers as well. These results for the first time reveal (1) pdaA as a phase-variable contingency locus in the adaptive evolution of endospore properties and (2) bet-hedging between what appears to be a quantity versus quality trade-off in endospore crops.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esporas Bacterianas / Bacillus cereus Idioma: En Revista: Curr Biol Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esporas Bacterianas / Bacillus cereus Idioma: En Revista: Curr Biol Asunto de la revista: BIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Reino Unido