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Developing portable and controllable fluorescence capillary imprinted sensor for visual detection Crohn's disease biomarkers.
Zhou, Qin; Wang, Xiangni; Tang, Kangling; Chen, Yu; Wang, Ruoyan; Lei, Huibin; Yang, Zhaoxia; Zhang, Zhaohui.
Afiliación
  • Zhou Q; College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China; Ley Laboratory of Medicinal Resources Chemistry and Pharmacology in Wuling Mountainous of Hunan Province College, Jishou University, Jishou, 416000, PR China.
  • Wang X; College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China; Ley Laboratory of Medicinal Resources Chemistry and Pharmacology in Wuling Mountainous of Hunan Province College, Jishou University, Jishou, 416000, PR China.
  • Tang K; College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China.
  • Chen Y; College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China.
  • Wang R; College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China; Ley Laboratory of Medicinal Resources Chemistry and Pharmacology in Wuling Mountainous of Hunan Province College, Jishou University, Jishou, 416000, PR China.
  • Lei H; College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China.
  • Yang Z; College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China.
  • Zhang Z; College of Chemistry and Chemical Engineering, Jishou University, Jishou, 416000, PR China; Ley Laboratory of Medicinal Resources Chemistry and Pharmacology in Wuling Mountainous of Hunan Province College, Jishou University, Jishou, 416000, PR China. Electronic address: zhaohuizhang77@163.com.
Talanta ; 278: 126402, 2024 Oct 01.
Article en En | MEDLINE | ID: mdl-38924985
ABSTRACT
Simultaneous detection of multiple biomarker levels is essential to improve the accuracy of early diagnosis. Introducing capillary will simplify procedure, less time, and reduce reagent consumption for point-of-care testing of biomarkers. Here, we developed a portable and controllable smartphone-integrated fluorescence capillary imprinted sensing platform for the accuracy visual detection of Crohn's disease biomarkers (lysozyme, Fe3+) using single-excitation/double-signal detection. A novel controllable capillary coating strategy was developed by static gas-driven coating method for synthesis uniform fluorescence capillary imprinted sensor (Si-CD/g-CdTe@MIP capillary sensor). When Fe3+ and lysozyme were added, the fluorescence intensity of Si-CD/g-CdTe@MIP capillary sensor was quenched at 426 nm and enhanced at 546 nm, respectively. This Si-CD/g-CdTe@MIP capillary sensor has high sensitivity and selectivity for quantification lysozyme and Fe3+ simultaneously with the detection limit of 0.098 nM and 0.20 nM, respectively. In addition, the smartphone-integrated Si-CD/g-CdTe@MIP capillary sensor was applied for the intelligent detection of lysozyme and Fe3+, in which the detection limit was calculated as 0.32 nM and 0.65 nM. The smartphone-integrated visual Si-CD/g-CdTe@MIP capillary sensor realized ultrasensitive microanalysis (18 µL/time) of biomarkers in health man and Crohn 's patients, providing a novel strategy for early diagnosis of Crohn 's disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores / Enfermedad de Crohn / Muramidasa Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores / Enfermedad de Crohn / Muramidasa Límite: Humans Idioma: En Revista: Talanta Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos