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Comparison of biofilm models for producing artificial active white spot lesions.
Araujo, Erika Michele Dos Santos; Vidal, Cristina de Mattos Pimenta; Zhu, Min; Banas, Jeffrey A; Freitas, Anderson Zanardi de; Wetter, Niklaus Ursus; Matos, Adriana Bona.
Afiliación
  • Araujo EMDS; The University of Iowa, College of Dentistry, Iowa Institute for Oral Health Research,Iowa City, IA, USA.
  • Vidal CMP; Universidade de São Paulo, Faculdade de Odontologia, Departamento de Dentística, São Paulo, SP, Brasil.
  • Zhu M; The University of Iowa, College of Dentistry, Department of Operative Dentistry, Iowa City, IA, USA.
  • Banas JA; The University of Iowa, College of Dentistry, Iowa Institute for Oral Health Research,Iowa City, IA, USA.
  • Freitas AZ; The University of Iowa, College of Dentistry, Iowa Institute for Oral Health Research,Iowa City, IA, USA.
  • Wetter NU; The University of Iowa, College of Dentistry, Department of Pediatric Dentistry, Iowa City, IA, USA.
  • Matos AB; Universidade de São Paulo, Instituto de Pesquisas Energéticas e Nucleares (IPEN), Centro de Lasers e Aplicações, São Paulo, SP, Brasil.
J Appl Oral Sci ; 32: e20230458, 2024.
Article en En | MEDLINE | ID: mdl-38922241
ABSTRACT

OBJECTIVE:

This study compared three protocols for developing artificial white spot lesions (WSL) using biofilm models.

METHODOLOGY:

In total, 45 human enamel specimens were sterilized and allocated into three groups based on the biofilm model Streptococcus sobrinus and Lactobacillus casei (Ss+Lc), Streptococcus sobrinus (Ss), or Streptococcus mutans (Sm). Specimens were incubated in filter-sterilized human saliva to form the acquired pellicle and then subjected to the biofilm challenge consisting of three days of incubation with bacteria (for demineralization) and one day of remineralization, which was performed once for Ss+Lc (four days total), four times for Ss (16 days total), and three times for Sm (12 days total). After WSL creation, the lesion fluorescence, depth, and chemical composition were assessed using Quantitative Light-induced Fluorescence (QLF), Polarized Light Microscopy (PLM), and Raman Spectroscopy, respectively. Statistical analysis consisted of two-way ANOVA followed by Tukey's post hoc test (α=0.05). WSL created using the Ss+Lc protocol presented statistically significant higher fluorescence loss (ΔF) and integrated fluorescence (ΔQ) in comparison to the other two protocols (p<0.001).

RESULTS:

In addition, Ss+Lc resulted in significantly deeper WSL (137.5 µm), followed by Ss (84.1 µm) and Sm (54.9 µm) (p<0.001). While high mineral content was observed in sound enamel surrounding the WSL, lesions created with the Ss+Lc protocol showed the highest demineralization level and changes in the mineral content among the three protocols.

CONCLUSION:

The biofilm model using S. sobrinus and L. casei for four days was the most appropriate and simplified protocol for developing artificial active WSL with lower fluorescence, higher demineralization, and greater depth.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptococcus mutans / Biopelículas / Caries Dental / Esmalte Dental / Lacticaseibacillus casei Límite: Humans Idioma: En Revista: J Appl Oral Sci Asunto de la revista: ODONTOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptococcus mutans / Biopelículas / Caries Dental / Esmalte Dental / Lacticaseibacillus casei Límite: Humans Idioma: En Revista: J Appl Oral Sci Asunto de la revista: ODONTOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Brasil