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Interference of Streptococcus agalactiae Blitz Therapy in Staphylococcus aureus Microbiological Diagnosis in Subclinical Bovine Mastitis.
Gomes, Ana Flávia Novaes; de Castro, Fúlvia de Fátima Almeida; Silva, Márcio Roberto; Lange, Carla Christine; Ribeiro, João Batista; Guimarães, Alessandro de Sá; de Souza, Guilherme Nunes.
Afiliación
  • Gomes AFN; Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Juiz de Fora 36038-330, Brazil.
  • de Castro FFA; Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Juiz de Fora 36038-330, Brazil.
  • Silva MR; Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Juiz de Fora 36038-330, Brazil.
  • Lange CC; Brazilian Agricultural Research Corporation, Juiz de Fora 36038-330, Brazil.
  • Ribeiro JB; Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Juiz de Fora 36038-330, Brazil.
  • Guimarães AS; Brazilian Agricultural Research Corporation, Juiz de Fora 36038-330, Brazil.
  • de Souza GN; Faculty of Pharmacy, Universidade Federal de Juiz de Fora, Juiz de Fora 36038-330, Brazil.
Vet Sci ; 11(6)2024 May 22.
Article en En | MEDLINE | ID: mdl-38921980
ABSTRACT
Bovine mastitis is an important and costly disease to dairy cattle. Diagnostic methods usually performed in Brazil are somatic cell counts (SCC) and milk microbiology. Low bacteria shedding in milk implies no colony growth in microbiological tests and false negative results. Streptococcus agalactiae and Staphylococcus aureus are principal pathogens of mixed mastitis. However, S. agalactiae has a higher bacterial release from the mammary gland than S. aureus, affecting microbiological sensitivity to diagnose S. aureus. This study aimed to estimate the SCC and total bacterial count (TBC) from cows according to pathogen isolated in milk and to evaluate variation in S. aureus diagnosis by a microbiological test during S. agalactiae treatment, which is called blitz therapy. Both S. agalactiae and S. aureus presented high SCC means, although S. agalactiae showed shedding of bacteria 2.3 times greater than S. aureus. Microbiological sensitivity to S. aureus increased for 5 months during S. agalactiae treatment. The prevalence of S. agalactiae fell after 5 months of therapeutic procedures. The prevalence of S. aureus increased to 39.0. The results showed that due to high sensitivity, the polymerase chain reaction (PCR) could be used at the beginning of blitz therapy with the goal of S. agalactiae eradication from the dairy herd.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet Sci Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet Sci Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza