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Four novel Acinetobacter lwoffii strains isolated from the milk of cows in China with subclinical mastitis.
Chen, Qiang; Zhou, Wensi; Cheng, Yuening; Wang, Guisheng; San, Zhihao; Guo, Li; Liu, Liming; Zhao, Cuiqing; Sun, Na.
Afiliación
  • Chen Q; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China.
  • Zhou W; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China.
  • Cheng Y; Key Laboratory of Special Animal Epidemic Disease, Ministry of Agriculture, Institute of Special Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun, China.
  • Wang G; Shandong Provincial Center for Animal Disease Control and Prevention, Jinan, China.
  • San Z; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China.
  • Guo L; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China.
  • Liu L; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China.
  • Zhao C; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China.
  • Sun N; College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin, China. sn111231@126.com.
BMC Vet Res ; 20(1): 274, 2024 Jun 26.
Article en En | MEDLINE | ID: mdl-38918815
ABSTRACT

BACKGROUND:

Acinetobacter lwoffii (A. lwoffii) is a Gram-negative bacteria common in the environment, and it is the normal flora in human respiratory and digestive tracts. The bacteria is a zoonotic and opportunistic pathogen that causes various infections, including nosocomial infections. The aim of this study was to identify A. lwoffii strains isolated from bovine milk with subclinical mastitis in China and get a better understanding of its antimicrobial susceptibility and resistance profile. This is the first study to analyze the drug resistance spectrum and corresponding mechanisms of A. lwoffii isolated in raw milk.

RESULTS:

Four A. lwoffii strains were isolated by PCR method. Genetic evolution analysis using the neighbor-joining method showed that the four strains had a high homology with Acinetobacter lwoffii. The strains were resistant to several antibiotics and carried 17 drug-resistance genes across them. Specifically, among 23 antibiotics, the strains were completely susceptible to 6 antibiotics, including doxycycline, erythromycin, polymyxin, clindamycin, imipenem, and meropenem. In addition, the strains showed variable resistance patterns. A total of 17 resistance genes, including plasmid-mediated resistance genes, were detected across the four strains. These genes mediated resistance to 5 classes of antimicrobials, including beta-lactam, aminoglycosides, fluoroquinolones, tetracycline, sulfonamides, and chloramphenicol.

CONCLUSION:

These findings indicated that multi-drug resistant Acinetobacter lwoffii strains exist in raw milk of bovine with subclinical mastitis. Acinetobacter lwoffii are widespread in natural environmental samples, including water, soil, bathtub, soap box, skin, pharynx, conjunctiva, saliva, gastrointestinal tract, and vaginal secretions. The strains carry resistance genes in mobile genetic elements to enhance the spread of these genes. Therefore, more attention should be paid to epidemiological surveillance and drug resistant A. lwoffii.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acinetobacter / Leche / Mastitis Bovina / Antibacterianos Límite: Animals País/Región como asunto: Asia Idioma: En Revista: BMC Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acinetobacter / Leche / Mastitis Bovina / Antibacterianos Límite: Animals País/Región como asunto: Asia Idioma: En Revista: BMC Vet Res Asunto de la revista: MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido