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Shape of the first mitotic spindles impacts multinucleation in human embryos.
Ono, Yuki; Shirasawa, Hiromitsu; Takahashi, Kazumasa; Goto, Mayumi; Ono, Takahiro; Sakaguchi, Taichi; Okabe, Motonari; Hirakawa, Takeo; Iwasawa, Takuya; Fujishima, Akiko; Sugawara, Tae; Makino, Kenichi; Miura, Hiroshi; Fukunaga, Noritaka; Asada, Yoshimasa; Kumazawa, Yukiyo; Terada, Yukihiro.
Afiliación
  • Ono Y; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan. onoy@med.akita-u.ac.jp.
  • Shirasawa H; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Takahashi K; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Goto M; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Ono T; Department of Neurosurgery, Akita University Graduate School of Medicine, Akita, Japan.
  • Sakaguchi T; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Okabe M; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Hirakawa T; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Iwasawa T; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Fujishima A; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Sugawara T; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Makino K; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Miura H; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Fukunaga N; Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Nagoya, Aichi, Japan.
  • Asada Y; Asada Institute for Reproductive Medicine, Asada Ladies Clinic, Nagoya, Aichi, Japan.
  • Kumazawa Y; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
  • Terada Y; Department of Obstetrics and Gynecology, Akita University Graduate School of Medicine, Akita, Japan.
Nat Commun ; 15(1): 5381, 2024 Jun 25.
Article en En | MEDLINE | ID: mdl-38918406
ABSTRACT
During human embryonic development, early cleavage-stage embryos are more susceptible to errors. Studies have shown that many problems occur during the first mitosis, such as direct cleavage, chromosome segregation errors, and multinucleation. However, the mechanisms whereby these errors occur during the first mitosis in human embryos remain unknown. To clarify this aspect, in the present study, we image discarded living human two-pronuclear stage zygotes using fluorescent labeling and confocal microscopy without microinjection of DNA or mRNA and investigate the association between spindle shape and nuclear abnormality during the first mitosis. We observe that the first mitotic spindles vary, and low-aspect-ratio-shaped spindles tend to lead to the formation of multiple nuclei at the 2-cell stage. Moreover, we observe defocusing poles in many of the first mitotic spindles, which are strongly associated with multinucleation. Additionally, we show that differences in the positions of the centrosomes cause spindle abnormality in the first mitosis. Furthermore, many multinuclei are modified to form mononuclei after the second mitosis because the occurrence of pole defocusing is firmly reduced. Our study will contribute markedly to research on the occurrence of mitotic errors during the early cleavage of human embryos.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Celular / Mitosis / Huso Acromático Límite: Female / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Celular / Mitosis / Huso Acromático Límite: Female / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido