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Genetic factors of grain cadmium concentration in Polish wheat (Triticum polonicum L.).
Cheng, Yiran; Liu, Rui; Yang, Tian; Yang, Shan; Chen, Jia; Huang, Yiwen; Long, Dan; Zeng, Jian; Wu, Dandan; Kang, Houyang; Fan, Xing; Sha, Lina; Zhang, Haiqin; Zhou, Yonghong; Wang, Yi.
Afiliación
  • Cheng Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Liu R; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Yang T; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Yang S; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Chen J; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Huang Y; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Long D; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Zeng J; College of Resources, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Wu D; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Kang H; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Fan X; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Sha L; Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Zhang H; College of Grassland Science and Technology, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Zhou Y; College of Grassland Science and Technology, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
  • Wang Y; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
Plant Physiol ; 196(2): 979-995, 2024 Oct 01.
Article en En | MEDLINE | ID: mdl-38917222
ABSTRACT
Wheat (Triticum aestivum L.) is one of the most important crops worldwide and a major source of human cadmium (Cd) intake. Limiting grain Cd concentration (Gr_Cd_Conc) in wheat is necessary to ensure food safety. However, the genetic factors associated with Cd uptake, translocation and distribution and Gr_Cd_Conc in wheat are poorly understood. Here, we mapped quantitative trait loci (QTLs) for Gr_Cd_Conc and its related transport pathway using a recombinant inbred line (RIL) population derived from 2 Polish wheat varieties (RIL_DT; dwarf Polish wheat [DPW] and tall Polish wheat [TPW]). We identified 29 novel major QTLs for grain and tissue Cd concentration; 14 novel major QTLs for Cd uptake, translocation, and distribution; and 27 major QTLs for agronomic traits. We also analyzed the pleiotropy of these QTLs. Six novel QTLs (QGr_Cd_Conc-1A, QGr_Cd_Conc-3A, QGr_Cd_Conc-4B, QGr_Cd_Conc-5B, QGr_Cd_Conc-6A, and QGr_Cd_Conc-7A) for Gr_Cd_Conc explained 8.16% to 17.02% of the phenotypic variation. QGr_Cd_Conc-3A, QGr_Cd_Conc-6A, and QGr_Cd_Conc-7A pleiotropically regulated Cd transport; 3 other QTLs were organ-specific for Gr_Cd_Conc. We fine-mapped the locus of QGr_Cd_Conc-4B and identified the candidate gene as Cation/Ca exchanger 2 (TpCCX2-4B), which was differentially expressed in DPW and TPW. It encodes an endoplasmic reticulum membrane/plasma membrane-localized Cd efflux transporter in yeast. Overexpression of TpCCX2-4B reduced Gr_Cd_Conc in rice. The average Gr_Cd_Conc was significantly lower in TpCCX2-4BDPW genotypes than in TpCCX2-4BTPW genotypes of the RIL_DT population and 2 other natural populations, based on a Kompetitive allele-specific PCR marker derived from the different promoter sequences between TpCCX2-4BDPW and TpCCX2-4BTPW. Our study reveals the genetic mechanism of Cd accumulation in wheat and provides valuable resources for genetic improvement of low-Cd-accumulating wheat cultivars.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triticum / Cadmio / Sitios de Carácter Cuantitativo Idioma: En Revista: Plant Physiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Triticum / Cadmio / Sitios de Carácter Cuantitativo Idioma: En Revista: Plant Physiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos