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Role of inflammation in a rat model of radiation retinopathy.
Lebon, Cécile; Malaise, Denis; Rimbert, Nicolas; Billet, Manon; Ramasamy, Gabriel; Villaret, Jérémie; Pouzoulet, Frédéric; Matet, Alexandre; Behar-Cohen, Francine.
Afiliación
  • Lebon C; Centre de Recherche des Cordeliers, INSERM UMRS 1138, Sorbonne Université, Physiopathology of Ocular Diseases: Therapeutic Innovations, 15 rue de l'école de Médecine, Paris, 75006, France.
  • Malaise D; Centre de Recherche des Cordeliers, INSERM UMRS 1138, Sorbonne Université, Physiopathology of Ocular Diseases: Therapeutic Innovations, 15 rue de l'école de Médecine, Paris, 75006, France.
  • Rimbert N; Ocular Oncology Department, Institut Curie, 26 rue d'Ulm, Paris, 75005, France.
  • Billet M; Centre de Recherche des Cordeliers, INSERM UMRS 1138, Sorbonne Université, Physiopathology of Ocular Diseases: Therapeutic Innovations, 15 rue de l'école de Médecine, Paris, 75006, France.
  • Ramasamy G; Translational Research Department, Experimental Radiotherapy Platform, Institut Curie, Université Paris Saclay, 15 Rue Georges Clemenceau, Orsay, 91400, France.
  • Villaret J; Translational Research Department, Experimental Radiotherapy Platform, Institut Curie, Université Paris Saclay, 15 Rue Georges Clemenceau, Orsay, 91400, France.
  • Pouzoulet F; Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts, Paris, 75012, France.
  • Matet A; Centre Rétine Gallien, Bordeaux, 33000, France.
  • Behar-Cohen F; Translational Research Department, Experimental Radiotherapy Platform, Institut Curie, Université Paris Saclay, 15 Rue Georges Clemenceau, Orsay, 91400, France.
J Neuroinflammation ; 21(1): 162, 2024 Jun 24.
Article en En | MEDLINE | ID: mdl-38915029
ABSTRACT
Radiation retinopathy (RR) is a major side effect of ocular tumor treatment by plaque brachytherapy or proton beam therapy. RR manifests as delayed and progressive microvasculopathy, ischemia and macular edema, ultimately leading to vision loss, neovascular glaucoma, and, in extreme cases, secondary enucleation. Intravitreal anti-VEGF agents, steroids and laser photocoagulation have limited effects on RR. The role of retinal inflammation and its contribution to the microvascular damage occurring in RR remain incompletely understood. To explore cellular and vascular events after irradiation, we analyzed their time course at 1 week, 1 month and 6 months after rat eyes received 45 Gy X-beam photons. Müller glial cells, astrocytes and microglia were rapidly activated, and these markers of retinal inflammation persisted for 6 months after irradiation. This was accompanied by early cell death in the outer retina, which persisted at later time points, leading to retinal thinning. A delayed loss of small retinal capillaries and retinal hypoxia were observed after 6 months, indicating inner blood‒retinal barrier (BRB) alteration but without cell death in the inner retina. Moreover, activated microglial cells invaded the entire retina and surrounded retinal vessels, suggesting the role of inflammation in vascular alteration and in retinal cell death. Radiation also triggered early and persistent invasion of the retinal pigment epithelium by microglia and macrophages, contributing to outer BRB disruption. This study highlights the role of progressive and long-lasting inflammatory mechanisms in RR development and demonstrates the relevance of this rat model to investigate human pathology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Modelos Animales de Enfermedad Límite: Animals Idioma: En Revista: J Neuroinflammation Asunto de la revista: NEUROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Modelos Animales de Enfermedad Límite: Animals Idioma: En Revista: J Neuroinflammation Asunto de la revista: NEUROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido