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HY5 and PIF antagonistically regulate HMGR expression and sterol biosynthesis in Arabidopsis thaliana.
Michael, Rahul; Ranjan, Avriti; Gautam, Swati; Trivedi, Prabodh Kumar.
Afiliación
  • Michael R; CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow 226001, India; Academy of Scientific and Innovative Research (AcSIR), CSIR, Ghaziabad 201002, India.
  • Ranjan A; Academy of Scientific and Innovative Research (AcSIR), CSIR, Ghaziabad 201002, India; Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Picnic Spot Road, Lucknow 226015, India.
  • Gautam S; CSIR-National Botanical Research Institute (CSIR-NBRI), Rana Pratap Marg, Lucknow 226001, India; Academy of Scientific and Innovative Research (AcSIR), CSIR, Ghaziabad 201002, India.
  • Trivedi PK; Academy of Scientific and Innovative Research (AcSIR), CSIR, Ghaziabad 201002, India; Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Picnic Spot Road, Lucknow 226015, India. Electronic address: prabodht@nbri.res.in.
Plant Sci ; 346: 112168, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38914157
ABSTRACT
Secondary metabolites play multiple crucial roles in plants by modulating various regulatory networks. The biosynthesis of these compounds is unique to each species and is intricately controlled by a range of developmental and environmental factors. While light's role in certain secondary metabolites is evident, its impact on sterol biosynthesis remains unclear. Previous studies indicate that ELONGATED HYPOCOTYL5 (HY5), a bZIP transcription factor, is pivotal in skotomorphogenesis to photomorphogenesis transition. Additionally, PHYTOCHROME INTERACTING FACTORs (PIFs), bHLH transcription factors, act as negative regulators. To unveil the light-dependent regulation of the mevalonic acid (MVA) pathway, a precursor for sterol biosynthesis, mutants of light signaling components, specifically hy5-215 and the pifq quadruple mutant (pif 1,3,4, and 5), were analyzed in Arabidopsis thaliana. Gene expression analysis in wild-type and mutants implicates HY5 and PIFs in regulating sterol biosynthesis genes. DNA-protein interaction analysis confirms their interaction with key genes like AtHMGR2 in the rate-limiting pathway. Results strongly suggest HY5 and PIFs' pivotal role in light-dependent MVA pathway regulation, including the sterol biosynthetic branch, in Arabidopsis, highlighting a diverse array of light signaling components finely tuning crucial growth pathways.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esteroles / Arabidopsis / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico / Factores de Transcripción con Cremalleras de Leucina de Carácter Básico Idioma: En Revista: Plant Sci Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esteroles / Arabidopsis / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico / Factores de Transcripción con Cremalleras de Leucina de Carácter Básico Idioma: En Revista: Plant Sci Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda