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Prokaryotic RNA N1-Methyladenosine Erasers Maintain tRNA m1A Modification Levels in Streptomyces venezuelae.
Foo, Marcus; Frietze, Luke R; Enghiad, Behnam; Yuan, Yujie; Katanski, Christopher D; Zhao, Huimin; Pan, Tao.
Afiliación
  • Foo M; Committee on Microbiology, The University of Chicago, Chicago, Illinois 60637, United States.
  • Frietze LR; Department of Biochemistry & Molecular Biology,, The University of Chicago, Chicago, Illinois60637, United States.
  • Enghiad B; Department of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61820, United States.
  • Yuan Y; Department of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61820, United States.
  • Katanski CD; Department of Biochemistry & Molecular Biology,, The University of Chicago, Chicago, Illinois60637, United States.
  • Zhao H; Department of Chemistry, Biochemistry, and Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61820, United States.
  • Pan T; Committee on Microbiology, The University of Chicago, Chicago, Illinois 60637, United States.
ACS Chem Biol ; 19(7): 1616-1625, 2024 Jul 19.
Article en En | MEDLINE | ID: mdl-38912606
ABSTRACT
tRNA modifications help maintain tRNA structure and facilitate translation and stress response. Found in all three kingdoms of life, m1A tRNA modification occurs in the T loop of many tRNAs, stabilizes tertiary tRNA structure, and impacts translation. M1A in the T loop is reversible by three mammalian demethylase enzymes, which bypasses the need of turning over the tRNA molecule to adjust its m1A levels in cells. However, no prokaryotic tRNA demethylase enzyme has been identified that acts on endogenous RNA modifications. Using Streptomyces venezuelae as a model organism, we confirmed the presence and quantitative m1A tRNA signatures using mass spectrometry and high-throughput tRNA sequencing. We identified two RNA demethylases that can remove m1A in tRNA and validated the activity of a previously annotated tRNA m1A writer. Using single-gene knockouts of these erasers and the m1A writer, we found dynamic changes of m1A levels in many tRNAs under stress conditions. Phenotypic characterization highlighted changes in their growth and altered antibiotic production. Our identification of the first prokaryotic tRNA demethylase enzyme paves the way for investigating new mechanisms of translational regulation in bacteria.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / ARN de Transferencia / Adenosina País/Región como asunto: America do sul / Venezuela Idioma: En Revista: ACS Chem Biol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Streptomyces / ARN de Transferencia / Adenosina País/Región como asunto: America do sul / Venezuela Idioma: En Revista: ACS Chem Biol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos