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Proteomics of Penicillium chrysogenum for a Deeper Understanding of Lead (Pb) Metal Bioremediation.
Algahmadi, Amjad; Mohammed, Afrah E; Alfadda, Assim A; Alanazi, Ibrahim O; Alwehaibi, Moudi A; Scaria Joy, Salini; Al-Shaye, Dalal; Benabdelkamel, Hicham.
Afiliación
  • Algahmadi A; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia.
  • Mohammed AE; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia.
  • Alfadda AA; Proteomics Resource Unit, Obesity Research Center and the Department of Medicine, College of Medicine, King Saud University, P O Box 2925 98 Riyadh 11461, Saudi Arabia.
  • Alanazi IO; Healthy Aging Research Institute Health Sector, King Abdulaziz City for Science and Technology (KACST), P O Box 6086 Riyadh 11442, Saudi Arabia.
  • Alwehaibi MA; Proteomics Resource Unit, Obesity Research Center, College of Medicine, King Saud University, P O Box 2925 98 Riyadh 11461, Saudi Arabia.
  • Scaria Joy S; Strategic Center for Diabetes Research, College of Medicine, King Saud University, Riyadh 12211, Saudi Arabia.
  • Al-Shaye D; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia.
  • Benabdelkamel H; Proteomics Resource Unit, Obesity Research Center, College of Medicine, King Saud University, P O Box 2925 98 Riyadh 11461, Saudi Arabia.
ACS Omega ; 9(24): 26245-26256, 2024 Jun 18.
Article en En | MEDLINE | ID: mdl-38911750
ABSTRACT
Penicillium chrysogenum (P. chrysogenum), a ubiquitous filamentous fungus, has demonstrated remarkable potential in the bioremediation of lead-contaminated environments. Its inherent tolerance and bioaccumulation capacity for lead (Pb), coupled with its relatively rapid growth rate, make it an attractive candidate for bioremediation applications. This study aims to identify the proteomic changes in P. chrysogenuminduced by Pb metal stress and unravel the roles of identified proteins in molecular mechanisms and cellular responses. Untargeted proteomic analysis was carried out using a two-dimensional difference in gel electrophoresis (2D-DIGE) coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). This study reported the identification of 43 statistically significant proteins (24 upregulated and 19 downregulated, ANOVA, p ≤ 0.05; fold change ≥1.5) in P. chrysogenum as a consequence of Pb treatment. Proteins were grouped according to their function into 18 groups from which 13 proteins were related to metabolism, 11 were related to cellular process and signaling, and 19 proteins were related to information storage and processing. The current study is considered the first report about the proteomics study of P. chrysogenum under Pb stress conditions, where upregulated proteins could better explain the mechanism of tolerance and Pb toxicity removal. Our research has provided a thorough understanding of the molecular and cellular processes involved in fungal-metal interactions, paving the way for the development of innovative molecular markers for heavy metal myco-remediation. To the best of our knowledge, this study of P. chrysogenum provides valuable insights toward growing research in comprehending the metal-microbe interactions. This will facilitate development of novel molecular markers for metal bioremediation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Omega Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ACS Omega Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Estados Unidos