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Microfragmented abdominal adipose tissue-derived stem cells from knee osteoarthritis patients aged 29-65 years demonstrate in vitro stemness and low levels of cellular senescence.
Hammer, Freja Aabæk; Hölmich, Per; Nehlin, Jan O; Vomstein, Kilian; Blønd, Lars; Hölmich, Lisbet Rosenkrantz; Barfod, Kristoffer Weisskirchner; Bagge, Jasmin.
Afiliación
  • Hammer FA; Sports Orthopedic Research Center-Copenhagen (SORC-C), Department of Orthopedic Surgery Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark.
  • Hölmich P; Sports Orthopedic Research Center-Copenhagen (SORC-C), Department of Orthopedic Surgery Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark.
  • Nehlin JO; Department of Clinical Research Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark.
  • Vomstein K; Department of Obstetrics and Gynecology, The Fertility Clinic Copenhagen University Hospital-Hvidovre Hvidovre Denmark.
  • Blønd L; Department of Orthopedic Surgery Zealand University Hospital-Køge Køge Denmark.
  • Hölmich LR; Department of Plastic Surgery Copenhagen University Hospital-Herlev and Gentofte Herlev Denmark.
  • Barfod KW; Sports Orthopedic Research Center-Copenhagen (SORC-C), Department of Orthopedic Surgery Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark.
  • Bagge J; Sports Orthopedic Research Center-Copenhagen (SORC-C), Department of Orthopedic Surgery Copenhagen University Hospital-Amager and Hvidovre Hvidovre Denmark.
J Exp Orthop ; 11(3): e12056, 2024 Jul.
Article en En | MEDLINE | ID: mdl-38911188
ABSTRACT

Purpose:

To investigate the level of cellular senescence in stem cells derived from microfragmented abdominal adipose tissue harvested from patients with knee osteoarthritis (OA).

Methods:

Stem cells harvested from microfragmented abdominal adipose tissue from 20 patients with knee OA, aged 29-65 years (mean = 49.8, SD = 9.58), were analysed as a function of patient age and compared with control cells exhibiting signs of cellular senescence. Steady-state mRNA levels of a panel of genes associated with senescence were measured by qPCR. Intracellular senescence-associated proteins p16 and p21, and senescence-associated ß-galactosidase activity were measured by flow cytometry. Cellular proliferation was assessed using a 5-ethynyl-2'-deoxyuridine proliferation assay. Stemness was assessed by stem cell surface markers using flow cytometry and the capacity to undergo adipogenic and osteogenic differentiation in vitro.

Results:

No correlation was found between cellular senescence levels of the microfragmented adipose tissue-derived stem cells and patient age for any of the standard assays used to quantify senescence. The level of cellular senescence was generally low across all senescence-associated assays compared to the positive senescence control. Stemness was verified for all samples. An increased capacity to undergo adipogenic differentiation was shown with increasing patient age (p = 0.02). No effect of patient age was found for osteogenic differentiation.

Conclusions:

Autologous microfragmented adipose tissue-derived stem cells may be used in clinical trials of knee OA of patients aged 29-65 years, at least until passage 4, as they show stemness potential and negligible senescence in vitro. Level of Evidence Not applicable.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Exp Orthop Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Exp Orthop Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos