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A novel long non-coding RNA linc-93.2 participates in bisphenol induced oxidative stress and macrophage polarization in red common carp (Cyprinus carpio).
Yu, Ting; Wei, Qing; Tang, Yiran; Cai, Ling; Chen, Bei; Yang, Ming.
Afiliación
  • Yu T; School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China; Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China.
  • Wei Q; School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
  • Tang Y; School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
  • Cai L; Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China. Electronic address: cailing@tio.org.cn.
  • Chen B; Fisheries Research Institute of Fujian, Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Xiamen, 361013, China.
  • Yang M; School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China. Electronic address: mingyang@shu.edu.cn.
Fish Shellfish Immunol ; 151: 109716, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38909636
ABSTRACT
Previous studies show that bisphenol A (BPA) and its analogs induce oxidative stress and promote inflammatory response. However, the key molecules in regulating this process remain unclear. Here, we report significant inductive effects of BPA and bisphenol AF (BPAF) on a newly found long non-coding RNA linc-93.2 accompanied by oxidative stress and activation of pro-inflammatory pathways in treated fish and fish primary macrophages. Silencing linc-93.2 in fish primary macrophages in vitro or fish in vivo significantly promotes the expression of anti-oxidative stress-related genes and anti-inflammatory cytokines. This inhibition of pro-inflammatory cytokine expression, showing cell status disruption towards to M2 polarization. Followed by exposure to BPA or BPAF, silencing linc-93.2 in vitro or in vivo significantly attenuates the increased production of reactive oxygen species and malondialdehyde level aroused by bisphenol treatment, possibly owing to the enhancement of total antioxidant capacity observed in cells and tissue after linc-93.2 knockdown. RNA-sequencing further revealed regulation of nuclear factor-kappa b (NF-κB) in linc-93.2's downstream network, combining with our previous observation on the upstream regulation of linc-93.2 via NF-κB, which together suggest a critical role of linc-93.2 in promoting NF-κB positive feedback loop that may be an important molecular event initiating the immunotoxicity of bisphenols.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenoles / Compuestos de Bencidrilo / Carpas / Estrés Oxidativo / ARN Largo no Codificante / Macrófagos Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenoles / Compuestos de Bencidrilo / Carpas / Estrés Oxidativo / ARN Largo no Codificante / Macrófagos Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido