Lipid-Based Nanoparticles for RNA Delivery.

Calderón-Colón, Xiomara; Egan, Richard

Calderón-Colón, Xiomara; Egan, Richard.

Afiliación

Calderón-Colón X; Asymmetric Operations Sector, Applied Biological Sciences, The Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA. Xiomara.Calderon-Colon@jhuapl.edu.
Egan R; Asymmetric Operations Sector, Applied Biological Sciences, The Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA.

Methods Mol Biol ; 2822: 411-416, 2024.

Article en En | MEDLINE | ID: mdl-38907931

ABSTRACT

ABSTRACT

Various nanoparticle-based delivery systems have been developed for the encapsulation and protection of active cargoes. Lipid nanoparticles represent one of the most widely used nanoparticle-based delivery systems for in vitro and in vivo applications, especially for the delivery of ribonucleic acid (RNA). In this chapter, a simple bulk mixing method for the encapsulation of RNA is described along with characterization techniques for measuring encapsulation efficiency and nanoparticle physicochemical properties.

Asunto(s)

Lípidos; Nanopartículas; ARN; Nanopartículas/química; ARN/química; Lípidos/química; Tamaño de la Partícula; Liposomas

Palabras clave

Bulk mixing method; Delivery; Lipid nanoparticles; Particle size; Physicochemical properties; Polydispersity index; Ribonucleic acid; Zeta potential

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Nanopartículas / Lípidos Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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