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Single-Cell Imaging of mRNA by Target RNA-Initiated RCA.
Ren, Xiaojun; Wu, Yifan; Deng, Ruijie; Li, Jinghong.
Afiliación
  • Ren X; Department of Chemistry, College of Chemistry and Life Science, Beijing University of Technology, Beijing, China.
  • Wu Y; Department of Chemistry, College of Chemistry and Life Science, Beijing University of Technology, Beijing, China.
  • Deng R; College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu, China.
  • Li J; Beijing Life Science Academy, Beijing, China. jhli@mail.tsinghua.edu.cn.
Methods Mol Biol ; 2822: 65-75, 2024.
Article en En | MEDLINE | ID: mdl-38907912
ABSTRACT
We present a powerful method for direct mRNA detection based on ligation-based recognition and in situ amplification, capable of single-cell imaging mRNA at single-nucleotide and single-molecule resolution. Attributed to the use of Splint R ligase that can ligate padlock probe with RNA as target template, this method can efficiently detect mRNA in the absence of reverse transcription. This method enables spatial localization and correlation analysis of gene expression in single cells, which helps us to elucidate gene function and regulatory mechanisms.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Mensajero / Análisis de la Célula Individual Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Mensajero / Análisis de la Célula Individual Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos