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Investigating the three-dimensional myocardial micro-architecture in the laminar structure using X-ray phase-contrast microtomography.
Wang, Shunli; Wang, Yan; Li, Zhaorui; Zhao, Yifei; Zhang, Ying; Varray, François.
Afiliación
  • Wang S; School of Instrumentation Science and Engineering, Harbin Institute of Technology (HIT), Harbin, 150080, China. m.wangshunli@hit.edu.cn.
  • Wang Y; Department of Medical Engineering, Strategic Support Force Xingcheng Special Duty Sanatorium, Xingcheng, 125100, China.
  • Li Z; School of Instrumentation Science and Engineering, Harbin Institute of Technology (HIT), Harbin, 150080, China.
  • Zhao Y; System Engineering Research Department, Beijing Institute of Aerospace Automatic Controls, Beijing, 100070, China.
  • Zhang Y; System Engineering Research Department, Beijing Institute of Aerospace Automatic Controls, Beijing, 100070, China.
  • Varray F; Université de Lyon, CREATIS, CNRS UMR5220, Inserm U1206, INSA-Lyon, Université Lyon 1, 69100, Villeurbanne, France.
Sci Rep ; 14(1): 14329, 2024 06 21.
Article en En | MEDLINE | ID: mdl-38907041
ABSTRACT
A comprehensive grasp of the myocardial micro-architecture is essential for understanding diverse heart functions. This study aimed to investigate three-dimensional (3D) cardiomyocyte arrangement in the laminar structure using X-ray phase-contrast microtomography. Using the ID-19 beamline at the European Synchrotron Radiation Facility, we imaged human left ventricular (LV) wall transparietal samples and reconstructed them with an isotropic voxel edge length of 3.5 µm. From the reconstructed volumes, we extracted different regions to analyze the orientation distribution of local cardiomyocyte aggregates, presenting findings in terms of helix and intrusion angles. In regions containing one sheetlet population, we observed cardiomyocyte aggregates running along the local LV wall's radial direction at the border of sheetlets, branching and merging into a complex network around connecting points of different sheetlets, and bending to accommodate vessel passages. In regions with two sheetlet populations, the helix angle of local cardiomyocyte aggregates experiences a nonmonotonic change, and some cardiomyocyte aggregates run along the local radial direction. X-ray phase-contrast microtomography is a valuable technique for investigating the 3D local myocardial architecture at microscopic level. The arrangement of local cardiomyocyte aggregates in the LV wall proves to be both regional and complex, intricately linked to the local laminar structure.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagenología Tridimensional / Miocitos Cardíacos / Microtomografía por Rayos X / Ventrículos Cardíacos Límite: Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagenología Tridimensional / Miocitos Cardíacos / Microtomografía por Rayos X / Ventrículos Cardíacos Límite: Humans Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido