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Automation of 3D digital rolling circle amplification using a 3D-printed liquid handler.
Shin, Suyeon; Yun, Hyo Geun; Chung, Haerim; Cho, Hyunsoo; Choi, Sungyoung.
Afiliación
  • Shin S; Department of Electronic Engineering, Hanyang University, Seoul, 04763, Republic of Korea.
  • Yun HG; Department of Electronic Engineering, Hanyang University, Seoul, 04763, Republic of Korea.
  • Chung H; Division of Hematology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea.
  • Cho H; Division of Hematology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, 03722, Republic of Korea. Electronic address: hyunsoocho@yuhs.ac.
  • Choi S; Department of Electronic Engineering, Hanyang University, Seoul, 04763, Republic of Korea; Department of Biomedical Engineering, Hanyang University, Seoul, 04763, Republic of Korea; Department of Healthcare Digital Engineering, Hanyang University, Seoul, 04763, Republic of Korea. Electronic address:
Biosens Bioelectron ; 261: 116503, 2024 Oct 01.
Article en En | MEDLINE | ID: mdl-38905856
ABSTRACT
Automation of liquid handling is indispensable to improve throughput and reproducibility in biochemical assays. However, the incorporation of automated systems into laboratory workflows is often hindered by the high cost and complexity associated with building robotic liquid handlers. Here, we report a 3D-printed liquid handler based on a fluidic manifold, thereby obviating the need for complex robotic mechanisms. The fluidic manifold, termed a dispensing and aspirating (DA) device, comprises parallelized multi-pipette structures connected by distribution and aspiration channels, enabling the precise supply and removal of reagents, respectively. Leveraging the versatility of 3D printing, the DA device can be custom-designed and printed to fit specific applications. As a proof-of-principle, we engineered a 3D-printed liquid handler dedicated for 3D digital rolling circle amplification (4DRCA), an advanced biochemical assay involving multiple sample preparation steps such as antibody incubation, cell fixation, nucleic acid amplification, probe hybridization, and extensive washing. We demonstrate the efficacy of the 3D-printed liquid handler to automate the preparation of clinical samples for the simultaneous, in situ analysis of oncogenic protein and transcript markers in B-cell acute lymphoblastic leukemia cells using 4DRCA. This approach provides an effective and accessible solution for liquid handling automation, offering high throughput and reproducibility in biochemical assays.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Técnicas de Amplificación de Ácido Nucleico / Impresión Tridimensional Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Técnicas de Amplificación de Ácido Nucleico / Impresión Tridimensional Límite: Humans Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido