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Glycyrrhiza uralensis polysaccharides ameliorates cecal ligation and puncture-induced sepsis by inhibiting the cGAS-STING signaling pathway.
Hui, Siwen; Kan, Wen; Qin, Shuanglin; He, Ping; Zhao, Jia; Li, Hui; Bai, Jun; Wen, Jincai; Mou, Wenqing; Hou, Manting; Wei, Ziying; Lin, Li; Xiao, Xiaohe; Xu, Guang; Bai, Zhaofang.
Afiliación
  • Hui S; Department of Hepatology, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Kan W; China Military Institute of Chinese Materia, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Qin S; School of Pharmacy, Xianning Medical College, Hubei University of Science and Technology, Xianning, China.
  • He P; Department of Hepatology, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Zhao J; China Military Institute of Chinese Materia, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Li H; Department of Hepatology, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Bai J; China Military Institute of Chinese Materia, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Wen J; School of Pharmacy, Xianning Medical College, Hubei University of Science and Technology, Xianning, China.
  • Mou W; Department of Hepatology, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Hou M; China Military Institute of Chinese Materia, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Wei Z; Department of Hepatology, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Lin L; China Military Institute of Chinese Materia, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Xiao X; Department of Hepatology, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Xu G; China Military Institute of Chinese Materia, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
  • Bai Z; Department of Neurosurgery, General Hospital of Chinese People Liberty Army, Beijing, China.
Front Pharmacol ; 15: 1374179, 2024.
Article en En | MEDLINE | ID: mdl-38904004
ABSTRACT
Ethnopharmacological relevance G. uralensis Fisch. (Glycyrrhiza uralensis) is an ancient and widely used traditional Chinese medicine with good efficacy in clearing heat and detoxifying action. Studies suggest that Glycyrrhiza Uralensis Polysaccharides (GUP), one of the major components of G. uralensis, has anti-inflammatory, anti-cancer and hepatoprotective effects., but its exact molecular mechanism has not been explored in depth. Aim of the study Objectives of our research are about exploring the anti-inflammatory role of GUP and the mechanisms of its action. Materials and

methods:

ELISA kits, Western blotting, immunofluorescence, quantitative real-time PCR, immunoprecipitation and DMXAA-mediated STING activation mice models were performed to investigate the role of GUP on the cGAS-STING pathway. To determine the anti-inflammatory effects of GUP, cecal ligation and puncture (CLP) sepsis models were employed.

Results:

GUP could effectively inhibit the activation of the cGAS-STING signaling pathway accompany by a decrease the expression of type I interferon-related genes and inflammatory factors in BMDMs, THP-1, and human PBMCs. Mechanistically, GUP does not affect the oligomerization of STING, but affects the interaction of STING with TBK1 and TBK1 with IRF3. Significantly, GUP had great therapeutic effects on DMXAA-induced agonist experiments in vivo as well as CLP sepsis in mice.

Conclusion:

Our studies suggest that GUP is an effective inhibitor of the cGAS-STING pathway, which may be a potential medicine for the treatment of inflammatory diseases mediated by the cGAS-STING pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Pharmacol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza