Antifungal, anti-biofilm, and anti-hyphal properties of <i>N</i>-substituted phthalimide derivatives against <i>Candida</i> species.

Shaik, Shamshe; Lee, Jin-Hyung; Kim, Yong-Guy; Lee, Jintae

Antifungal, anti-biofilm, and anti-hyphal properties of N-substituted phthalimide derivatives against Candida species.

Shaik, Shamshe; Lee, Jin-Hyung; Kim, Yong-Guy; Lee, Jintae.

Afiliación

Shaik S; School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea.
Lee JH; School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea.
Kim YG; School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea.
Lee J; School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea.

Front Cell Infect Microbiol ; 14: 1414618, 2024.

Article en En | MEDLINE | ID: mdl-38903941

ABSTRACT

ABSTRACT

Candida species comprise a ubiquitous pathogenic fungal genus responsible for causing candidiasis. They are one of the primary causatives of several mucosal and systemic infections in humans and can survive in various environments. In this study, we investigated the antifungal, anti-biofilm, and anti-hyphal effects of six N-substituted phthalimides against three Candida species. Of the derivatives, N-butylphthalimide (NBP) was the most potent, with a minimum inhibitory concentration (MIC) of 100 µg/ml and which dose-dependently inhibited biofilm at sub-inhibitory concentrations (10-50 µg/ml) in both the fluconazole-resistant and fluconazole-sensitive Candida albicans and Candida parapsilosis. NBP also effectively inhibited biofilm formation in other pathogens including uropathogenic Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus, and Vibrio parahaemolyticus, along with the polymicrobial biofilms of S. epidermidis and C. albicans. NBP markedly inhibited the hyphal formation and cell aggregation of C. albicans and altered its colony morphology in a dose-dependent manner. Gene expression analysis showed that NBP significantly downregulated the expression of important hyphal- and biofilm-associated genes, i.e., ECE1, HWP1, and UME6, upon treatment. NBP also exhibited mild toxicity at concentrations ranging from 2 to 20 µg/ml in a nematode model. Therefore, this study suggests that NBP has anti-biofilm and antifungal potential against various Candida strains.

Asunto(s)

Antifúngicos; Biopelículas; Candida albicans; Hifa; Pruebas de Sensibilidad Microbiana; Ftalimidas; Biopelículas/efectos de los fármacos; Biopelículas/crecimiento & desarrollo; Antifúngicos/farmacología; Ftalimidas/farmacología; Candida albicans/efectos de los fármacos; Hifa/efectos de los fármacos; Hifa/crecimiento & desarrollo; Candida/efectos de los fármacos; Candidiasis/microbiología; Candidiasis/tratamiento farmacológico; Animales; Humanos; Candida parapsilosis/efectos de los fármacos; Proteínas Fúngicas/genética; Proteínas Fúngicas/metabolismo; Fluconazol/farmacología

Palabras clave

Candida; anti-fungal; biofilm; hyphae; phthalimide; polymicrobial

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ftalimidas / Candida albicans / Pruebas de Sensibilidad Microbiana / Biopelículas / Hifa / Antifúngicos Límite: Animals / Humans Idioma: En Revista: Front Cell Infect Microbiol Año: 2024 Tipo del documento: Article Pais de publicación: Suiza

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