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Phenotypic and Genotypic Responses of Foodborne Pathogens to Sublethal Concentrations of Lactic Acid and Sodium Chloride.
Dawan, Jirapat; Liao, Xinyu; Ding, Tian; Ahn, Juhee.
Afiliación
  • Dawan J; Department of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea.
  • Liao X; Future Food Laboratory, Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing, China.
  • Ding T; Future Food Laboratory, Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing, China.
  • Ahn J; Department of Food Science and Nutrition, Zhejiang University, Hangzhou, China.
Microb Drug Resist ; 30(8): 332-340, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38900709
ABSTRACT
The aim of this study was to evaluate the phenotypic and genotypic responses of Salmonella Typhimurium ATCC 19585 (ST) and Staphylococcus aureus KACC 13236 (SA) preadapted to sublethal concentrations of lactic acid (LA) and sodium chloride (NaCl) for 48 hr at 37°C, followed by re-exposure to lethal concentrations of LA and NaCl for 24 hr at 37°C. ST and SA treated in a sequential and ordered manner with LA and NaCl were assigned as LA-LA, LA-NaCl, NaCl-LA, and NaCl-NaCl. The treatments, LA-LA, LA-NaCl, NaCl-LA, and NaCl-NaCl, were evaluated by antimicrobial susceptibility, bacterial fluctuation, relative fitness, zeta potential, and gene expression. The MICt/MICc ratios of LA, NaCl, CIP, GEN, and TET against ST treated with LA-LA were 1.0 to 0.8, 0.8, 0.3, 0.4, and 0.5, respectively. The MICt/MICc ratios of NaCl, CIP, GEN, and TET were between 0.5-0.8 for SA treated with LA-LA. ST treated with LA-LA and SA treated with LA-NaCl exhibited the highest coefficient of variance. The lowest relative fitness was observed at ST treated with LA-LA (0.5). ST and SA treated with LA-LA showed the lowest zeta potential. The transporter-, toxin-antitoxin system-, chaperone protein-, and SOS response-related genes were suppressed at ST and SA treated with LA-LA. The transporter-, toxin-antitoxin system-, and chaperone protein-related genes were overexpressed in SA treated with LA-NaCl, NaCl-LA, and NaCl-NaCl. The results suggest that ST and SA treated with LA-LA, LA-NaCl, NaCl-LA, and NaCl-NaCl could induce collateral sensitivity and cross-resistance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Salmonella typhimurium / Staphylococcus aureus / Cloruro de Sodio / Pruebas de Sensibilidad Microbiana / Ácido Láctico / Genotipo / Antibacterianos Idioma: En Revista: Microb Drug Resist Asunto de la revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Salmonella typhimurium / Staphylococcus aureus / Cloruro de Sodio / Pruebas de Sensibilidad Microbiana / Ácido Láctico / Genotipo / Antibacterianos Idioma: En Revista: Microb Drug Resist Asunto de la revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos