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Widespread genomic de novo DNA methylation occurs following CD8+ T cell activation and proliferation.
Seddon, Annika R; Damiano, Olivia M; Hampton, Mark B; Stevens, Aaron J.
Afiliación
  • Seddon AR; Department of Pathology and Biomedical Science, Matai Haora - Centre for Redox Biology and Medicine, University of Otago, Christchurch, New Zealand.
  • Damiano OM; Department of Pathology and Molecular Medicine, Genetics and Epigenetics Research Group, University of Otago, Wellington, New Zealand.
  • Hampton MB; Department of Pathology and Biomedical Science, Matai Haora - Centre for Redox Biology and Medicine, University of Otago, Christchurch, New Zealand.
  • Stevens AJ; Department of Pathology and Molecular Medicine, Genetics and Epigenetics Research Group, University of Otago, Wellington, New Zealand.
Epigenetics ; 19(1): 2367385, 2024 Dec.
Article en En | MEDLINE | ID: mdl-38899429
ABSTRACT
This research investigates the intricate dynamics of DNA methylation in the hours following CD8+ T cell activation, during a critical yet understudied temporal window. DNA methylation is an epigenetic modification central to regulation of gene expression and directing immune responses. Our investigation spanned 96-h post-activation and unveils a nuanced tapestry of global and site-specific methylation changes. We identified 15,626 significant differentially methylated CpGs spread across the genome, with the most significant changes occurring within the genes ADAM10, ICA1, and LAPTM5. While many changes had modest effect sizes, approximately 120 CpGs exhibited a log2FC above 1.5, with cell activation and proliferation pathways the most affected. Relatively few of the differentially methylated CpGs occurred along adjacent gene regions. The exceptions were seven differentially methylated gene regions, with the Human T cell Receptor Alpha Joining Genes demonstrating consistent methylation change over a 3kb window. We also investigated whether an inflammatory environment could alter DNA methylation during activation, with proliferating cells exposed to the oxidant glycine chloramine. No substantial differential methylation was observed in this context. The temporal perspective of early activation adds depth to the evolving field of epigenetic immunology, offering insights with implications for therapeutic innovation and expanding our understanding of epigenetic modulation in immune function.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación de Linfocitos / Linfocitos T CD8-positivos / Metilación de ADN / Proliferación Celular Límite: Humans Idioma: En Revista: Epigenetics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Activación de Linfocitos / Linfocitos T CD8-positivos / Metilación de ADN / Proliferación Celular Límite: Humans Idioma: En Revista: Epigenetics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Estados Unidos