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A novel dual-signal output strategy for POCT of CEA based on a smartphone electrochemical aptasensing platform.
Shi, Shan-Shan; Li, Xiao-Jian; Ma, Rong-Na; Shang, Lei; Zhang, Wei; Zhao, Huai-Qing; Jia, Li-Ping; Wang, Huai-Sheng.
Afiliación
  • Shi SS; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong, 250022, People's Republic of China.
  • Li XJ; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, People's Republic of China.
  • Ma RN; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, People's Republic of China.
  • Shang L; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, People's Republic of China.
  • Zhang W; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, People's Republic of China.
  • Zhao HQ; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, People's Republic of China.
  • Jia LP; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong, 250022, People's Republic of China. chm_zhaohq@ujn.edu.cn.
  • Wang HS; College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252000, People's Republic of China. jialiping@lcu.edu.cn.
Mikrochim Acta ; 191(7): 407, 2024 06 19.
Article en En | MEDLINE | ID: mdl-38898338
ABSTRACT
A smartphone-based electrochemical aptasensing platform was developed for the point-of-care testing (POCT) of carcinoembryonic antigen (CEA) based on the ferrocene (Fc) and PdPt@PCN-224 dual-signal labeled strategy. The prepared PdPt@PCN-224 nanocomposite showed a strong catalytic property for the reduction of H2O2. Phosphate group-labeled aptamer could capture PdPt@PCN-224 by Zr-O-P bonds to form PdPt@PCN-224-P-Apt. Therefore, a dual signal labeled probe was formed by the hybridization between Fc-DNA and PdPt@PCN-224-P-Apt. The presence of CEA forced PdPt@PCN-224-P-Apt to leave the electrode surface due to the specific affinity, leading to the decrease of the reduction current of H2O2. At the same time, the Fc-DNA strand changed to hairpin structure, which made Fc closer to the electrode and resulted in the increase of the oxidation current of Fc. Thus, CEA can be accurately determined through both signals the decrease of H2O2 reduction current and the increase of Fc oxidation current, which could avoid the false positive signal. Under the optimal conditions, the prepared aptasensor exhibited a wide linear range from 1 pg·mL-1 to 100 ng·mL-1 and low detection limits of 0.98 pg·mL-1 and 0.27 pg·mL-1 with Fc and PdPt@PCN-224 as signal labels, respectively. The aptasensor developed in this study has successfully demonstrated its capability to detect CEA in real human serum samples. These findings suggest that the proposed sensing platform will hold great potential for clinical tumor diagnosis and monitoring.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paladio / Compuestos Ferrosos / Técnicas Biosensibles / Antígeno Carcinoembrionario / Aptámeros de Nucleótidos / Técnicas Electroquímicas / Límite de Detección / Teléfono Inteligente / Pruebas en el Punto de Atención / Peróxido de Hidrógeno Límite: Humans Idioma: En Revista: Mikrochim Acta Año: 2024 Tipo del documento: Article Pais de publicación: Austria
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paladio / Compuestos Ferrosos / Técnicas Biosensibles / Antígeno Carcinoembrionario / Aptámeros de Nucleótidos / Técnicas Electroquímicas / Límite de Detección / Teléfono Inteligente / Pruebas en el Punto de Atención / Peróxido de Hidrógeno Límite: Humans Idioma: En Revista: Mikrochim Acta Año: 2024 Tipo del documento: Article Pais de publicación: Austria