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UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium.
Palma, Leopoldo; Ruiz de Escudero, Iñigo; Mañeru-Oria, Francisco; Berry, Colin; Caballero, Primitivo.
Afiliación
  • Palma L; Instituto de Biotecnología y Biomedicina (BIOTECMED), Departamento de Genética, Universitat de València, 46100, Burjassot, Spain. Electronic address: leopoldo.palma@uv.es.
  • Ruiz de Escudero I; Institute of Multidisciplinary Research in Applied Biology-IMAB, Universidad Pública de Navarra, 31006, Pamplona, Spain.
  • Mañeru-Oria F; Institute of Multidisciplinary Research in Applied Biology-IMAB, Universidad Pública de Navarra, 31006, Pamplona, Spain.
  • Berry C; School of Biosciences, Cardiff University, Park Place, Cardiff, CF10 3AX, UK.
  • Caballero P; Institute of Multidisciplinary Research in Applied Biology-IMAB, Universidad Pública de Navarra, 31006, Pamplona, Spain; Departamento de Investigación y Desarrollo, Bioinsectis SL, Plaza Cein 5, Nave A14, 31110, Noáin, Spain. Electronic address: pcm92@unavarra.es.
Toxicon ; 247: 107807, 2024 Aug 28.
Article en En | MEDLINE | ID: mdl-38897358
ABSTRACT
In this study, secretable Vip3Ag4 protein was encapsulated in Bacillus megaterium and used for quantitative bioassays, in order to determine the UV photoprotective capacity of the cell, for preventing inactivation of the insecticidal activity of the protein. The non-encapsulated and purified protein was exposed to the UV light showing a LC50 of 518 ng/cm2 against Spodoptera littoralis larvae, whereas the exposed encapsulated protein exhibited 479 ng/cm2. In addition to the capability to accumulate Vip3 proteins for the development of novel insecticidal formulates, the B. megaterium cell has demonstrated to provide moderate protection against the deleterious action of UV light.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus megaterium / Proteínas Bacterianas / Rayos Ultravioleta / Spodoptera / Insecticidas Límite: Animals Idioma: En Revista: Toxicon Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacillus megaterium / Proteínas Bacterianas / Rayos Ultravioleta / Spodoptera / Insecticidas Límite: Animals Idioma: En Revista: Toxicon Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido