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The ROS/TXNIP/NLRP3 pathway mediates LPS-induced microglial inflammatory response.
Zhao, Qianlei; Liu, Guanhao; Ding, Qiang; Zheng, Feixia; Shi, Xulai; Lin, Zhongdong; Liang, Yafeng.
Afiliación
  • Zhao Q; Department of Pediatric Neurology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China.
  • Liu G; Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China.
  • Ding Q; Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China.
  • Zheng F; Department of Pediatric Neurology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China.
  • Shi X; Department of Pediatric Neurology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China.
  • Lin Z; Department of Pediatric Neurology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China.
  • Liang Y; Department of Pediatric Emergency, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China. Electronic address: lyafeng77@163.com.
Cytokine ; 181: 156677, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38896955
ABSTRACT

BACKGROUND:

Sepsis-associated encephalopathy (SAE) is a diffuse brain dysfunction activated by microglia. The potential pathological changes of SAE are complex, and the cellular pathophysiological characteristics remains unclear. This study aims to explore the ROS/TXNIP/NLRP3 pathway mediated lipopolysaccharide (LPS)-induced inflammatory response in microglia.

METHODS:

BV-2 cells were pre-incubated with 10 µM N-acetyl-L-cysteine (NAC) for 2 h, which were then reacted with 1 µg/mL LPS for 24 h. Western blot assay examined the protein levels of IBA1, CD68, TXNIP, NLRP3, ASC, and Cleaved Caspase-1 in BV-2 cells. The contents of inflammatory factor were detected by ELISA assay. The co-immunoprecipitation assay examined the interaction between TXNIP and NLRP3.

RESULTS:

LPS was confirmed to promote the positive expressions of IBA1 and CD68 in BV-2 cells. The further experiments indicated that LPS enhanced ROS production and NLRP3 inflammasome activation in BV-2 cells. Moreover, we also found that NAC partially reversed the facilitation of LPS on the levels of ROS, IL-1ß, IL-18, TXNIP, NLRP3, ASC, and Cleaved Caspase-1 in BV-2 cells. NAC treatment also notably alleviated the interaction between TXNIP and NLRP3 in BV-2 cells.

CONCLUSION:

ROS inhibition mediated NLRP3 signaling inactivation by decreasing TXNIP expression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Proteínas Portadoras / Lipopolisacáridos / Especies Reactivas de Oxígeno / Microglía / Caspasa 1 / Inflamasomas / Proteína con Dominio Pirina 3 de la Familia NLR / Inflamación Límite: Animals Idioma: En Revista: Cytokine Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Proteínas Portadoras / Lipopolisacáridos / Especies Reactivas de Oxígeno / Microglía / Caspasa 1 / Inflamasomas / Proteína con Dominio Pirina 3 de la Familia NLR / Inflamación Límite: Animals Idioma: En Revista: Cytokine Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido