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Antibody Production and Immunoassay Development for Authenticating Chlorpheniramine Maleate Adulteration in Herbal Tea.
Lin, Jianhao; Liu, Zhiwei; Guan, Tian; Lei, Yi; Pan, Liangwen; Yu, Xiaoqin; Zhang, Shiwei; Huang, Xin-An; Lei, Hongtao; Chen, Jiahong.
Afiliación
  • Lin J; Guangdong Provincial Key Laboratory of Food Quality and Safety/Nation-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, Guangzhou 510642, China.
  • Liu Z; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
  • Guan T; Guangdong Provincial Key Laboratory of Food Quality and Safety/Nation-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, Guangzhou 510642, China.
  • Lei Y; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
  • Pan L; Guangdong Provincial Key Laboratory of Food Quality and Safety/Nation-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, Guangzhou 510642, China.
  • Yu X; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
  • Zhang S; Guangzhou Institute of Food Inspection, Guangzhou 511410, China.
  • Huang XA; Animal, Plant and Food Inspection and Quarantine Technology Center, Shanghai Customs, Shanghai 200135, China.
  • Lei H; Sichuan Institute of Food Inspection, Chengdu 610097, China.
  • Chen J; National Nutrition Food Testing Center, Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518131, China.
Foods ; 13(11)2024 May 22.
Article en En | MEDLINE | ID: mdl-38890838
ABSTRACT
Chlorphenamine maleate is a prohibited additive found in herbal teas and health foods. Excessive intake of this substance can result in adverse health effects. In this study, two novel haptens, PEM and bepotastine (PB1), mimicking chlorphenamine maleate structure were designed and synthesized based on molecular simulation for developing two corresponding polyclonal antibodies (PEM-Ab and PB1-Ab), respectively. Afterward, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed to quickly and accurately detect chlorphenamine maleate in herbal teas using PB1-Ab, which has a high sensitivity and specificity. For chlorphenamine maleate, the half-maximal inhibitory concentration (IC50) and limit of detection (LOD) of PB1-Ab under ideal circumstances were found to be 1.18 µg/L and 0.07 µg/L, respectively. Besides, an environmentally friendly sample pre-treatment strategy was employed that allowed easy and effective elimination of complex matrices. The ic-ELISA method observed the average recovery rate from 87.7% to 94.0% with the variance coefficient (CV) ranging from 2.2% to 9.4%. Additionally, the identification of 25 commercially available herbal teas using liquid chromatography-tandem mass spectrometry (LC-MS/MS) further confirmed the validity of our detection. The results of the two methods are consistent. Overall, the proposed ic-ELISA could be an ultrasensitive and reliable method for chlorphenamine maleate adulterated in foods or exposure to the environment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Foods Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Foods Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza