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Exploring the effects of naringin on oxidative stress-impaired osteogenic differentiation via the Wnt/ß-catenin and PI3K/Akt pathways.
Wang, Hui; Liang, Jun; Wang, Yiran; Zheng, Junyuan; Liu, Ying; Zhao, Yiyang; Ma, Yixuan; Chen, Pei; Yang, Xufang.
Afiliación
  • Wang H; Department of Pathophysiology, Mudanjiang Medical University, No. 3 Tongxiang Road, Mudanjiang , 157011, Heilongjiang, China.
  • Liang J; Department of Morphology Laboratory, Mudanjiang Medical University, Mudanjiang, 157011, Heilongjiang, China.
  • Wang Y; The first Clinical Medicine College, Mudanjiang Medical University, NO. 3 Tongxiang Road, Mudanjiang, 157011, Heilongjiang, China.
  • Zheng J; Department of Pathophysiology, Mudanjiang Medical University, No. 3 Tongxiang Road, Mudanjiang , 157011, Heilongjiang, China.
  • Liu Y; Department of Foreign Languages, Mudanjiang Medical University, Mudanjiang, 157011, Heilongjiang, China.
  • Zhao Y; The First Clinical Medicine College, Southern Medical University, Guangzhou, 510000, Guangdong, China.
  • Ma Y; The First Clinical Medicine College, Harbin Medical University, Harbin, 150000, Heilongjiang, China.
  • Chen P; Department of Pathophysiology, Mudanjiang Medical University, No. 3 Tongxiang Road, Mudanjiang , 157011, Heilongjiang, China.
  • Yang X; Department of Pathophysiology, Mudanjiang Medical University, No. 3 Tongxiang Road, Mudanjiang , 157011, Heilongjiang, China. yangxufang12@163.com.
Sci Rep ; 14(1): 14047, 2024 06 18.
Article en En | MEDLINE | ID: mdl-38890371
ABSTRACT
This study aimed to explore naringin's potential to promote the osteogenic differentiation of MC3T3-E1 under oxidative stress. It delved into Nar's connection with the Wnt/ß-catenin and PI3K/Akt signaling pathways. Initially, 2911 OP-related genes were analyzed, revealing close ties with the PI3K/Akt and Wnt pathways alongside oxidative stress. Nar's potential targets-ESR1, HSP90AA1, and ESR2-were identified through various databases and molecular docking studies confirmed Nar's affinity with ESR1 and HSP90AA1. Experiments established optimal concentrations for Nar and H2O2. H2O2 at 0.3 mmol/L damaged MC3T3-E1 cells, alleviated by 0.1 µmol/L Nar. Successful establishment of oxidative stress models was confirmed by DCFH-DA probe and NO detection. Nar exhibited the ability to enhance osteogenic differentiation, counteracting oxidative damage. It notably increased osteoblast-related protein expression in MC3T3-E1 cells under oxidative stress. The study found Nar's positive influence on GSK-3ß phosphorylation, ß-catenin accumulation, and pathway-related protein expression, all critical in promoting osteogenic differentiation. The research concluded that Nar effectively promotes osteogenic differentiation in MC3T3-E1 cells under oxidative stress. It achieved this by activating the Wnt/ß-catenin and PI3K/Akt pathways, facilitating GSK-3ß phosphorylation, and enhancing ß-catenin accumulation, pivotal in osteogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Diferenciación Celular / Estrés Oxidativo / Fosfatidilinositol 3-Quinasas / Flavanonas / Proteínas Proto-Oncogénicas c-akt / Beta Catenina / Vía de Señalización Wnt Límite: Animals Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Diferenciación Celular / Estrés Oxidativo / Fosfatidilinositol 3-Quinasas / Flavanonas / Proteínas Proto-Oncogénicas c-akt / Beta Catenina / Vía de Señalización Wnt Límite: Animals Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido