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Changes in adiponectin levels of subclinical ketosis cows and their effects on steroid hormone secretion and proliferation in follicular granulosa cells.
Zhao, Chang; Jiang, BenZheng; Yan, Weizhe; Wang, Xichun; Ding, Hongyan; Xia, Cheng.
Afiliación
  • Zhao C; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Jiang B; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Yan W; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Wang X; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Ding H; Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, China. Electronic address: Xcwlxyf2014@163.com.
  • Xia C; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, China. Electronic address: Xcwlxyf2014@163.com.
Reprod Biol ; 24(3): 100898, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38889545
ABSTRACT
In dairy cows, the occurrence of subclinical ketosis (SCK) is particularly high during early lactation. Previously, we documented alterations in the abundance of adiponectin (ADPN) in anestrus cows with SCK in comparison to cows in estrus. In the present study, 60 cows were divided into two groups control (C, n = 30) and SCK (n = 30). Based on cow's estrus situation in two group at 55-60 days postpartum, 15 anestrus SCK cows and estrus cows were designated the SCK-A group and C-E group, respectively. The SCK-A group had downregulated serum and follicular fluid ADPN levels compared with the C-E group. The serum ADPN level was positively correlated with the insulin level and follicle growth rate, and there was a positive correlation between ADPN and glucose in the follicular fluid. Primary culture of dairy cow granulosa cells (GCs) was established to observe the effect of low glucose (Glu) and/or ADPN on GCs cyclins and proteins important for steroid synthesis. The results showed that the addition of 1 µg/mL ADPN alleviated the negative effects of low Glu treatment on the proliferation of GCs and the expression of steroid secretion related protein proteins. Treatment with LY294002 (PI3K inhibitor) four experimental GCs groups control (0 µg/mL ADPN), 1 µg/mL ADPN, LY294002 inhibitor, and 1 µg/mL ADPN+LY294002. The results showed that ADPN promotes the secretion of steroid hormones by GCs through the PI3K-AKT. In summary, ADPN plays a crucial role in ameliorating postpartum anestrus in dairy cows with SCK.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Bovinos / Líquido Folicular / Proliferación Celular / Adiponectina / Células de la Granulosa / Cetosis Límite: Animals Idioma: En Revista: Reprod Biol Asunto de la revista: MEDICINA REPRODUTIVA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Polonia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de los Bovinos / Líquido Folicular / Proliferación Celular / Adiponectina / Células de la Granulosa / Cetosis Límite: Animals Idioma: En Revista: Reprod Biol Asunto de la revista: MEDICINA REPRODUTIVA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Polonia