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Eµ-TCL1 adoptive transfer mouse model of chronic lymphocytic leukemia.
Fernandez Botana, Iria; Gonder, Susanne; Klapp, Vanessa; Moussay, Etienne; Paggetti, Jerome.
Afiliación
  • Fernandez Botana I; Tumor Stroma Interactions, Department of Cancer Research, Luxembourg Institute of Health, Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
  • Gonder S; Tumor Stroma Interactions, Department of Cancer Research, Luxembourg Institute of Health, Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
  • Klapp V; Tumor Stroma Interactions, Department of Cancer Research, Luxembourg Institute of Health, Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
  • Moussay E; Tumor Stroma Interactions, Department of Cancer Research, Luxembourg Institute of Health, Luxembourg, Luxembourg. Electronic address: etienne.moussay@lih.lu.
  • Paggetti J; Tumor Stroma Interactions, Department of Cancer Research, Luxembourg Institute of Health, Luxembourg, Luxembourg. Electronic address: jerome.paggetti@lih.lu.
Methods Cell Biol ; 188: 109-129, 2024.
Article en En | MEDLINE | ID: mdl-38880520
ABSTRACT
Despite being the most common adult leukemia in the western world, Chronic Lymphocytic Leukemia (CLL) remains a life-threatening and incurable disease. Efforts to develop new treatments are highly dependent on the availability of appropriate mouse models for pre-clinical testing. The Eµ-TCL1 mouse model is the most established pre-clinical approach to study CLL pathobiology and response to treatment, backed by numerous studies highlighting its resemblance to the most aggressive form of this malignancy. In contrast to the transgenic Eµ-TCL1 model, employing the adoptive transfer of Eµ-TCL1-derived splenocytes in immunocompetent C57BL/6 mice results in a comparably rapid (e.g., leukemic development within weeks compared to months in the transgenic model) and reliable model mimicking CLL. In this chapter, we would like to provide readers with a thoroughly optimized, detailed, and comprehensive protocol to use the adoptive transfer Eµ-TCL1 model in their research.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Linfocítica Crónica de Células B / Traslado Adoptivo / Modelos Animales de Enfermedad / Ratones Endogámicos C57BL Límite: Animals / Humans Idioma: En Revista: Methods Cell Biol Año: 2024 Tipo del documento: Article País de afiliación: Luxemburgo Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leucemia Linfocítica Crónica de Células B / Traslado Adoptivo / Modelos Animales de Enfermedad / Ratones Endogámicos C57BL Límite: Animals / Humans Idioma: En Revista: Methods Cell Biol Año: 2024 Tipo del documento: Article País de afiliación: Luxemburgo Pais de publicación: Estados Unidos