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Preliminary study on mitochondrial DNA analysis from different sports items.
Noor, Shehla; Akhtar, Sareen; Khan, Muhammad Farhan; Rehman, Rahat Abdul; Salman, Midhat; Nazir, Shahid; Rakha, Allah; Ullah, Inam; Munawar, Anam.
Afiliación
  • Noor S; University of Lahore, Pakistan.
  • Akhtar S; Xia'n Jiaotong University, China.
  • Khan MF; Department of Forensic Medicine/Medical Jurisprudence, University of Health Sciences, Lahore, Pakistan.
  • Rehman RA; Department of Forensic Medicine/Medical Jurisprudence, University of Health Sciences, Lahore, Pakistan.
  • Salman M; Department of Forensic Medicine/Medical Jurisprudence, University of Health Sciences, Lahore, Pakistan.
  • Nazir S; Department of Forensic Medicine/Medical Jurisprudence, University of Health Sciences, Lahore, Pakistan.
  • Rakha A; Department of Forensic Medicine/Medical Jurisprudence, University of Health Sciences, Lahore, Pakistan.
  • Ullah I; University of Lahore, Pakistan. Electronic address: inamullah@imbb.uol.edu.pk.
  • Munawar A; Department of Forensic Medicine/Medical Jurisprudence, University of Health Sciences, Lahore, Pakistan. Electronic address: anammunwar22@gmail.com.
Forensic Sci Int ; 361: 112077, 2024 Aug.
Article en En | MEDLINE | ID: mdl-38878615
ABSTRACT
Criminals often attempt to conceal blood-stained weapons used in violent crimes, making forensic evidence crucial in solving cases. This study explores the recovery and extraction of trace DNA from sports equipment, including cricket bats, table tennis racquets, and hockey sticks, which are frequently implicated in such incidents. Our research evaluates various double swab collection methods for retrieving trace DNA from these sports items, emphasizing those associated with blunt force trauma. We also compare presumptive and confirmatory tests to establish a direct correlation. This research consistently demonstrated robust DNA recovery, surpassing a 50 % threshold across all tests. Specifically, DNA recovery from buried samples reached an impressive 87 %, while washed samples still yielded a substantial 80 % efficiency. We conducted a comparative analysis between presumptive and confirmatory testing methods, establishing a direct correlation between the two. Variability in DNA recovery efficiency was observed and attributed to factors like the type of surface the items contacted, and ambient humidity levels. In addition to presenting robust DNA recovery rates, statistical analyses were employed to compare methods, establishing correlations and highlighting the influence of environmental factors on DNA recovery efficiency. These findings have significant implications for forensic investigations involving silent weapons crafted from sports equipment, emphasizing the need for standardized protocols and consideration of environmental factors in DNA analysis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Manejo de Especímenes / ADN Mitocondrial / Dermatoglifia del ADN Límite: Humans Idioma: En Revista: Forensic Sci Int Año: 2024 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Irlanda
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Manejo de Especímenes / ADN Mitocondrial / Dermatoglifia del ADN Límite: Humans Idioma: En Revista: Forensic Sci Int Año: 2024 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Irlanda