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Enhancing Proliferation of Stem Cells from Human Exfoliated Deciduous Teeth (SHED) through hTERT Expression while Preserving Stemness and Multipotency.
Yadav, Pooja; Vats, Ravina; Wadhwa, Sapna; Bano, Afsareen; Namdev, Ritu; Gupta, Monika; Bhardwaj, Rashmi.
Afiliación
  • Yadav P; Centre for Medical Biotechnology, Maharshi Dayanand University Rohtak, Rohtak, Haryana, 124001, India.
  • Vats R; Centre for Medical Biotechnology, Maharshi Dayanand University Rohtak, Rohtak, Haryana, 124001, India.
  • Wadhwa S; Centre for Medical Biotechnology, Maharshi Dayanand University Rohtak, Rohtak, Haryana, 124001, India.
  • Bano A; Centre for Medical Biotechnology, Maharshi Dayanand University Rohtak, Rohtak, Haryana, 124001, India.
  • Namdev R; Dept. of Pediatric Dentistry, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, 124001, India.
  • Gupta M; Dept. of Pathology, Post Graduate Institute of Medical Sciences, Rohtak, Haryana, 124001, India.
  • Bhardwaj R; Centre for Medical Biotechnology, Maharshi Dayanand University Rohtak, Rohtak, Haryana, 124001, India. bhardwajrashmi3@gmail.com.
Stem Cell Rev Rep ; 2024 Jun 15.
Article en En | MEDLINE | ID: mdl-38878252
ABSTRACT

BACKGROUND:

Stem cells from human exfoliated deciduous teeth (SHED) hold promise in regenerative medicine owing to their multipotent capabilities resembling mesenchymal stem cells (MSCs). Despite their potential, SHED have not been extensively investigated because their limited lifespan and unavailability of cell-lines pose challenges for therapeutic applications. This study investigated the effect of ectopic human telomerase reverse transcriptase (hTERT) expression on SHEDs' proliferation while preserving stemness and genomic integrity.

METHODS:

Deciduous teeth were collected from children aged 6-10 years. After isolation and characterization, the SHED were transduced with pBabe-puro-hTERT retrovirus to establish SHED cell-line, which was evaluated and compared with pBabe-puro (mock control) for stemness, multipotency and growth attributes through flow cytometry, trilineage differentiation, and growth kinetics. We also estimated hTERT gene expression, genomic integrity, and validated cell-line through STR analysis.

RESULTS:

Following hTERT transduction, SHED displayed elevated hTERT gene expression while retaining fibroblast-like morphology and mesenchymal stem cell markers. Moreover, after hTERT transduction cellular shape remained same along with increased replicative lifespan and proliferation potential. SHED-hTERT cells exhibited multi-potency and maintained stemness, as evidenced by surface marker expression and multilineage differentiation. Furthermore, genomic integrity was not affected by hTERT integration, as confirmed by STR analysis and CDKN2A gene assessment.

CONCLUSION:

Ectopic hTERT expression in SHED successfully prolonged their replicative lifespan and improved their ability to proliferate and migrate, while preserving their stemness, multipotency and genomic integrity, suggesting minimal carcinogenic risk. Establishment of SHED cell-line holds potential in regenerative medicine applications, especially in cell-based drugs and tissue engineering experiments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Stem Cell Rev Rep Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Stem Cell Rev Rep Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos