Your browser doesn't support javascript.
loading
A Reversible Neural Stem Cell Quiescence and Activation Culture System for Metabolic Study.
Hu, Ke; Jin, Shengkai; Yue, Ke; Wang, Huan; Cai, Chunhui; Liu, Qian; Guo, Jianrong; Liang, Qiujuan; Tian, Yu; Gao, Zhengliang.
Afiliación
  • Hu K; Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Nantong, China.
  • Jin S; Department of Anesthesiology, Shanghai Gongli Hospital, Naval Military Medical University, Shanghai, China.
  • Yue K; Fundamental Research Center, Shanghai YangZhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), School of Medicine, Tongji University, Shanghai, China.
  • Wang H; China-Japan Friendship Medical Research Institute, School of Medicine, Shanghai University, Shanghai, China.
  • Cai C; Institute of Geriatrics (Shanghai University), Affiliated Nantong Hospital of Shanghai University (The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Nantong, China.
  • Liu Q; Department of Anesthesiology, Shanghai Gongli Hospital, Naval Military Medical University, Shanghai, China.
  • Guo J; Department of Anesthesiology, Shanghai Gongli Hospital, Naval Military Medical University, Shanghai, China.
  • Liang Q; China-Japan Friendship Medical Research Institute, School of Medicine, Shanghai University, Shanghai, China.
  • Tian Y; Department of Anesthesiology, Shanghai Gongli Hospital, Naval Military Medical University, Shanghai, China.
  • Gao Z; Fundamental Research Center, Shanghai YangZhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), School of Medicine, Tongji University, Shanghai, China.
Cell Transplant ; 33: 9636897241259723, 2024.
Article en En | MEDLINE | ID: mdl-38877676
ABSTRACT
Stem cells in vivo can transit between quiescence and activation, two metabolically distinct states. It is increasingly appreciated that cell metabolism assumes profound roles in stem cell maintenance and tissue homeostasis. However, the lack of suitable models greatly hinders our understanding of the metabolic control of stem cell quiescence and activation. In the present study, we have utilized classical signaling pathways and developed a cell culture system to model reversible NSC quiescence and activation. Unlike activated ones, quiescent NSCs manifested distinct morphology characteristics, cell proliferation, and cell cycle properties but retained the same cell proliferation and differentiation potentials once reactivated. Further transcriptomic analysis revealed that extensive metabolic differences existed between quiescent and activated NSCs. Subsequent experimentations confirmed that NSC quiescence and activation transition was accompanied by a dramatic yet coordinated and dynamic shift in RNA metabolism, protein synthesis, and mitochondrial and autophagy activity. The present work not only showcases the broad utilities of this powerful in vitro NSC quiescence and activation culture system but also provides timely insights for the field and warrants further investigations.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Proliferación Celular / Células-Madre Neurales Límite: Animals Idioma: En Revista: Cell Transplant Asunto de la revista: TRANSPLANTE Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Diferenciación Celular / Proliferación Celular / Células-Madre Neurales Límite: Animals Idioma: En Revista: Cell Transplant Asunto de la revista: TRANSPLANTE Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos