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CryoEM reveals the structure of an archaeal pilus involved in twitching motility.
Gaines, Matthew C; Sivabalasarma, Shamphavi; Isupov, Michail N; Haque, Risat Ul; McLaren, Mathew; Hanus, Cyril; Gold, Vicki A M; Albers, Sonja-Verena; Daum, Bertram.
Afiliación
  • Gaines MC; Living Systems Institute, University of Exeter, Exeter, UK.
  • Sivabalasarma S; Department of Biosciences, Faculty of Health and Life Sciences, Exeter, UK.
  • Isupov MN; Institute of Biology, Molecular Biology of Archaea, University of Freiburg, Freiburg, Germany.
  • Haque RU; Spemann Graduate School of Biology and Medicine, University of Freiburg, Freiburg, Germany.
  • McLaren M; Henry Wellcome Building for Biocatalysis, Department of Biosciences, Faculty of Health and Life Sciences, University of Exeter, Exeter, UK.
  • Hanus C; Living Systems Institute, University of Exeter, Exeter, UK.
  • Gold VAM; Department of Biosciences, Faculty of Health and Life Sciences, Exeter, UK.
  • Albers SV; Living Systems Institute, University of Exeter, Exeter, UK.
  • Daum B; Department of Biosciences, Faculty of Health and Life Sciences, Exeter, UK.
Nat Commun ; 15(1): 5050, 2024 Jun 14.
Article en En | MEDLINE | ID: mdl-38877033
ABSTRACT
Amongst the major types of archaeal filaments, several have been shown to closely resemble bacterial homologues of the Type IV pili (T4P). Within Sulfolobales, member species encode for three types of T4P, namely the archaellum, the UV-inducible pilus system (Ups) and the archaeal adhesive pilus (Aap). Whereas the archaellum functions primarily in swimming motility, and the Ups in UV-induced cell aggregation and DNA-exchange, the Aap plays an important role in adhesion and twitching motility. Here, we present a cryoEM structure of the Aap of the archaeal model organism Sulfolobus acidocaldarius. We identify the component subunit as AapB and find that while its structure follows the canonical T4P blueprint, it adopts three distinct conformations within the pilus. The tri-conformer Aap structure that we describe challenges our current understanding of pilus structure and sheds new light on the principles of twitching motility.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfolobus acidocaldarius / Microscopía por Crioelectrón Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sulfolobus acidocaldarius / Microscopía por Crioelectrón Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido