Serum proteinogram of gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax) as a new useful approach for detecting loss of haemostasis.

Campos-Sánchez, Jose Carlos; Guardiola, Francisco A; Esteban, María Ángeles

Campos-Sánchez, Jose Carlos; Guardiola, Francisco A; Esteban, María Ángeles.

Afiliación

Campos-Sánchez JC; Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain.
Guardiola FA; Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain.
Esteban MÁ; Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, 30100, Murcia, Spain. Electronic address: aesteban@um.es.

Fish Shellfish Immunol ; 151: 109699, 2024 Aug.

Article en En | MEDLINE | ID: mdl-38876410

ABSTRACT

ABSTRACT

Proteinograms, a semiquantitative analytical method that separates proteins into multiple bands, have not been explored in teleosts for diagnostic or prognostic purposes. This study aimed to establish reference values for proteinograms in the serum of gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax), two important farmed fish species in the Mediterranean region. Serum proteins were studied using SDS-PAGE, electropherogram, and HPLC-mass spectrometry. SDS-PAGE analysis revealed four major bands of proteins around 11, 25, 70, and 100 kDa in the serum of gilthead seabream and European sea bass. Electropherogram results showed that a protein with a molecular weight of 76.8 kDa was the most abundant protein in the serum of gilthead seabream, while a peak of 75.5 kDa was the most abundant in European sea bass. HPLC-mass spectrometry detected 87 proteins and 119 proteins in the serum of gilthead seabream and European sea bass, respectively, including α1-globulins, α2-globulins, ß-globulins, and Î³-globulins. Notably, the albumin sequence was not detected in either of the two species. These results help to characterize the serum protein profile and to establish reference proteinograms for these two fish species. They also provide a basis for the development of novel approaches for the rapid detection of loss of haemostasis due to stress, health disorders or disease in farmed fish.

Asunto(s)

Lubina; Proteínas Sanguíneas; Proteínas de Peces; Dorada; Animales; Lubina/sangre; Dorada/sangre; Proteínas Sanguíneas/análisis; Proteínas de Peces/sangre; Proteínas de Peces/química; Proteínas de Peces/genética; Hemostasis; Electroforesis en Gel de Poliacrilamida/veterinaria; Espectrometría de Masas/veterinaria; Valores de Referencia; Cromatografía Líquida de Alta Presión/veterinaria

Palabras clave

Aquaculture; Electropherogram; European seabass (Dicentrarchus labrax); Gilthead seabream (Sparus aurata); Serum proteinogram

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lubina / Proteínas Sanguíneas / Dorada / Proteínas de Peces Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2024 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido

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