Your browser doesn't support javascript.
loading
Comparison of polystyrene and hydrogel microcarriers for optical imaging of adherent cells.
Benavides, Oscar R; White, Berkley P; Gibbs, Holly C; Kaunas, Roland; Gregory, Carl A; Maitland, Kristen C; Walsh, Alex J.
Afiliación
  • Benavides OR; Texas A&M University, Department of Biomedical Engineering, College Station, Texas, United States.
  • White BP; Texas A&M University, Department of Biomedical Engineering, College Station, Texas, United States.
  • Gibbs HC; Texas A&M University, Department of Biomedical Engineering, College Station, Texas, United States.
  • Kaunas R; Texas A&M University, Microscopy and Imaging Center, College Station, Texas, United States.
  • Gregory CA; Texas A&M University, Department of Biomedical Engineering, College Station, Texas, United States.
  • Maitland KC; Texas A&M Health Science Center, School of Medicine, Bryan, Texas, United States.
  • Walsh AJ; Texas A&M University, Department of Biomedical Engineering, College Station, Texas, United States.
J Biomed Opt ; 29(Suppl 2): S22708, 2024 Jun.
Article en En | MEDLINE | ID: mdl-38872791
ABSTRACT

Significance:

The ability to observe and monitor cell density and morphology has been imperative for assessing the health of a cell culture and for producing high quality, high yield cell cultures for decades. Microcarrier-based cultures, used for large-scale cellular expansion processes, are not compatible with traditional visualization-based methods, such as widefield microscopy, due to their thickness and material composition.

Aim:

Here, we assess the optical imaging compatibilities of commercial polystyrene microcarriers versus custom-fabricated gelatin methacryloyl (gelMA) microcarriers for non-destructive and non-invasive visualization of the entire microcarrier surface, direct cell enumeration, and sub-cellular visualization of mesenchymal stem/stromal cells.

Approach:

Mie scattering and wavefront error simulations of the polystyrene and gelMA microcarriers were performed to assess the potential for elastic scattering-based imaging of adherent cells. A Zeiss Z.1 light-sheet microscope was adapted to perform light-sheet tomography using label-free elastic scattering contrast from planar side illumination to achieve optical sectioning and permit non-invasive and non-destructive, in toto, three-dimensional, high-resolution visualization of cells cultured on microcarriers.

Results:

The polystyrene microcarrier prevents visualization of cells on the distal half of the microcarrier using either fluorescence or elastic scattering contrast, whereas the gelMA microcarrier allows for high fidelity visualization of cell morphology and quantification of cell density using light-sheet fluorescence microscopy and tomography.

Conclusions:

The combination of optical-quality gelMA microcarriers and label-free light-sheet tomography will facilitate enhanced control of bioreactor-microcarrier cell culture processes.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Poliestirenos / Adhesión Celular / Hidrogeles / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: J Biomed Opt Asunto de la revista: ENGENHARIA BIOMEDICA / OFTALMOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Poliestirenos / Adhesión Celular / Hidrogeles / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: J Biomed Opt Asunto de la revista: ENGENHARIA BIOMEDICA / OFTALMOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos