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Increased expression of miR-320b in blood plasma of patients in response to SARS-CoV-2 infection.
de Souza Nicoletti, Aline; Berlofa Visacri, Marília; Regina da Silva Correa da Ronda, Carla; Tiemi Siguemoto, Julia; Motta Neri, Carolini; Nogueira de Souza, Rafael; de Souza Ventura, Deise; Eguti, Adriana; Ferreira de Souza Silva, Lilian; Wesley Perroud Junior, Mauricio; Buosi, Keini; Jalalizadeh, Mehrsa; Dionato, Franciele; Dal Col, Luciana; Giacomelli, Cristiane; Leme, Patrícia; Oliveira Reis, Leonardo; Augusto Dos Santos, Luiz; Durán, Nelson; José Fávaro, Wagner; Luiz da Costa, José; Dagli-Hernandez, Carolina; Moriel, Patricia; de Carvalho Pincinato, Eder.
Afiliación
  • de Souza Nicoletti A; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Berlofa Visacri M; School of Pharmaceutical Science, Universidade de São Paulo (USP), São Paulo, SP, Brazil.
  • Regina da Silva Correa da Ronda C; Faculty of Pharmaceutical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Tiemi Siguemoto J; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Motta Neri C; Faculty of Pharmaceutical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Nogueira de Souza R; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • de Souza Ventura D; Hospital Estadual de Sumaré Dr. Leandro Francheschini, Sumaré, SP, Brazil.
  • Eguti A; Hospital Estadual de Sumaré Dr. Leandro Francheschini, Sumaré, SP, Brazil.
  • Ferreira de Souza Silva L; Hospital Estadual de Sumaré Dr. Leandro Francheschini, Sumaré, SP, Brazil.
  • Wesley Perroud Junior M; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Buosi K; Hospital Estadual de Sumaré Dr. Leandro Francheschini, Sumaré, SP, Brazil.
  • Jalalizadeh M; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Dionato F; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Dal Col L; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Giacomelli C; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Leme P; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Oliveira Reis L; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Augusto Dos Santos L; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Durán N; School of Life Sciences, Pontifical Catholic University of Campinas (PUC-Campinas), Campinas, SP, Brazil.
  • José Fávaro W; Hospital Municipal de Paulínia, Paulínia, SP, Brazil.
  • Luiz da Costa J; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Dagli-Hernandez C; School of Medical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • Moriel P; Faculty of Pharmaceutical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
  • de Carvalho Pincinato E; Faculty of Pharmaceutical Science, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.
Sci Rep ; 14(1): 13702, 2024 06 14.
Article en En | MEDLINE | ID: mdl-38871789
ABSTRACT
Coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Recent research has demonstrated how epigenetic mechanisms regulate the host-virus interactions in COVID-19. It has also shown that microRNAs (miRNAs) are one of the three fundamental mechanisms of the epigenetic regulation of gene expression and play an important role in viral infections. A pilot study published by our research group identified, through next-generation sequencing (NGS), that miR-4433b-5p, miR-320b, and miR-16-2-3p are differentially expressed between patients with COVID-19 and controls. Thus, the objectives of this study were to validate the expression of these miRNAs using quantitative real-time polymerase chain reaction (qRT-PCR) and to perform in silico analyses. Patients with COVID-19 (n = 90) and healthy volunteers (n = 40) were recruited. MiRNAs were extracted from plasma samples and validated using qRT-PCR. In addition, in silico analyses were performed using mirPath v.3 software. MiR-320b was the only miRNA upregulated in the case group com-pared to the control group. The in silico analyses indicated the role of miR-320b in the regulation of the KITLG gene and consequently in the inflammatory process. This study confirmed that miR-320b can distinguish patients with COVID-19 from control participants; however, further research is needed to determine whether this miRNA can be used as a target or a biomarker.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / SARS-CoV-2 / COVID-19 Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / SARS-CoV-2 / COVID-19 Límite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido