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Primordial germ cells of Astyanax altiparanae, isolated and recovered intact after vitrification: A preliminary study for potential cryopreservation of Neotropical fish germplasm.
Rosero, Jenyffer; Pessoa, Giselle Pessanha; Carvalho, Gabriella Braga; López, Lucia Suárez; Dos Santos, Silvio Carlos Alves; Bressan, Fabiana Fernandes; Yasui, George Shigueki.
Afiliación
  • Rosero J; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil; Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity C
  • Pessoa GP; Institute of Bioscience, São Paulo State University, Botucatu, São Paulo, Brazil; Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil.
  • Carvalho GB; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil; Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity C
  • López LS; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil; Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity C
  • Dos Santos SCA; AES Tiête, Promissão, São Paulo, Brazil.
  • Bressan FF; Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, São Paulo, Brazil; Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, São Paulo, Brazil.
  • Yasui GS; Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil; Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Aquatic Biodiversity, Chico Mendes Institute of Biodiversity C
Cryobiology ; 116: 104929, 2024 Sep.
Article en En | MEDLINE | ID: mdl-38871206
ABSTRACT
Primordial germ cells (PGCs) constitute an important cell lineage that directly impacts genetic dissemination and species conservation through the creation of cryobanks. In order to advance the field of animal genetic cryopreservation, this work aimed to recover intact PGCs cryopreserved in embryonic tissues during the segmentation phase for subsequent in vitro maintenance, using the yellow-tailed tetra (Astyanax altiparanae) as a model organism. For this, a total of 202 embryos were distributed in two experiments. In the first experiment, embryos in the segmentation phase were dissociated, and isolated PGCs were maintained in vitro. They were visualized using gfp-Pm-ddx4 3'UTR labeling. The second experiment aimed to vitrify PGCs using 3 cryoprotective agents or CPAs (dimethyl sulfoxide, ethylene glycol, and 1,2 propanediol) at 3 molarities (2, 3, and 4 M). The toxicity, somatic cell viability, and recovery of intact PGCs were evaluated. After cryopreservation and thawing, 2 M ethylene glycol produced intact PGCs and somatic cells (44 ± 11.52 % and 42.35 ± 0.33 %, respectively) post-thaw. The recovery of PGCs from frozen embryonic tissues was not possible without the use of CPAs. Thus, the vitrification of PGCs from an important developmental model and Neotropical species such as A. altiparanae was achieved, and the process of isolating and maintaining PGCs in a culture medium was successful. Therefore, to ensure the maintenance of genetic diversity, PGCs obtained during embryonic development in the segmentation phase between 25 and 28 somites were stored through vitrification for future applications in the reconstitution of species through germinal chimerism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criopreservación / Crioprotectores / Vitrificación / Células Germinativas Límite: Animals Idioma: En Revista: Cryobiology Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criopreservación / Crioprotectores / Vitrificación / Células Germinativas Límite: Animals Idioma: En Revista: Cryobiology Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos